Human/Mouse FoxP3 PE-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC8214P
Recombinant Monoclonal Antibody. Use with Catalog # FC012, FlowX FoxP3 Fixation & Permeabilization Buffer Kit
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse
Applications
Intracellular Staining by Flow Cytometry
Label
Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1054C
Product Specifications
Immunogen
E. coli-derived recombinant human FoxP3
Met1-Leu71
Accession # Q9BZS1
Met1-Leu71
Accession # Q9BZS1
Specificity
Detects human and mouse FoxP3 in flow cytometry.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human/Mouse FoxP3 PE-conjugated Antibody
Detection of FoxP3 in Human PBMC Natural Tregs by Flow Cytometry.
Human peripheral blood mononuclear cell (PBMC) natural regulatory T cells (Tregs) were surface stained with (A) Mouse Anti-Human CD4 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB3791F) and (B) Mouse Anti-Human IL-2 Ra (CD25) APC-conjugated Monoclonal Antibody (Catalog # FAB1020A), followed by intracellular staining using Rabbit Anti-Human/Mouse FoxP3 PE-conjugated Monoclonal Antibody (Catalog # IC8214P). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). Cells were gated on lymphocytes.
Detection of FoxP3 in C57BL/6 Mouse Splenocyte Natural Tregs by Flow Cytometry.
C57BL/6 mouse splenocyte natural regulatory T cells (Tregs) were surface stained with (A) Rat Anti-Mouse CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB554A) and (B) Rat Anti-Mouse IL-2 Ra (CD25) APC-conjugated Monoclonal Antibody (Catalog # FAB2438A) followed by intracellular staining using Rabbit Anti-Human/Mouse FoxP3 PE-conjugated Monoclonal Antibody (Catalog # IC8214P). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). Cells were gated on lymphocytes.Applications for Human/Mouse FoxP3 PE-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Sample: Human peripheral blood mononuclear cell (PBMC) natural regulatory T cells (Tregs) and C57BL/6 mouse splenocyte natural regulatory T cells (Tregs) were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). Cells were gated on lymphocytes.
Sample: Human peripheral blood mononuclear cell (PBMC) natural regulatory T cells (Tregs) and C57BL/6 mouse splenocyte natural regulatory T cells (Tregs) were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). Cells were gated on lymphocytes.
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Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: FoxP3
Long Name
Forkhead Box P3
Alternate Names
AIID, DIETER, IPEX, JM2, PIDX, SCURFIN, XPID
Gene Symbol
FOXP3
UniProt
Additional FoxP3 Products
Product Documents for Human/Mouse FoxP3 PE-conjugated Antibody
Product Specific Notices for Human/Mouse FoxP3 PE-conjugated Antibody
For research use only
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