Species Reactivity:

Human, Mouse

Immunogen:

Mouse myeloma cell line NS0-derived recombinant mouse CD117/c-kit
Gln25-Thr519 (Ala207Glu)
Accession # P05532

Applications:

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Western Blot

Label:

Alexa Fluor 488 (Excitation=488 nm, Emission=515-545 nm)

Specificity:

Detects human and mouse CD117/c-kit in direct ELISAs and Western blots.

View Full Technical Details for Human/Mouse CD117/c-kit Alexa Fluor® 488-conjugated Antibody

Technical Data
Citations / Reviews

Applications

	Recommended Concentration	Sample
Western Blot	Optimal dilution of this antibody should be experimentally determined.
Flow Cytometry	Optimal dilution of this antibody should be experimentally determined.
Immunohistochemistry	Optimal dilution of this antibody should be experimentally determined.
CyTOF-ready	Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Formulation	Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD117/c-kit

Stem cell factor receptor (CD117, the gene product of the c-kit protooncogene) and its ligand, stem cell factor (also named c-kit ligand, mast cell growth factor), play essential roles in gametogenesis, melanogenesis and hematopoiesis. It is a transmembrane tyrosine kinase that is expressed on endothelial cells, mast cells, megakaryocytes, stem cells and multiple embryonic cells, such as melanoblasts and primordial germ cells.

Item	Value
Long Name	Stem Cell Factor Receptor
Entrez Gene IDs	3815 (Human); 16590 (Mouse); 64030 (Rat)
Alternate Names	CD117, CD117 antigen, EC 2.7.10, EC 2.7.10.1, KIT, PBT, Proto-oncogene c-Kit, SCF R, SCFR, SCFRmast/stem cell growth factor receptor, Tyrosine-protein kinase Kit, c-kit, ckit, piebald trait, proto-oncogene tyrosine-protein kinase Kit, soluble KIT variant 1, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

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Product Datasheets

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Human/Mouse CD117/c-kit Alexa Fluor® 488-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # AF1356G

Key Product Details