Species Reactivity:

Human, Mouse

Immunogen:

E. coli-derived recombinant human c-Myc
Arg66-Asp201
Accession # P01106

Applications:

Chromatin Immunoprecipitation, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Knockout Validated, Western Blot

Label:

Alexa Fluor 488 (Excitation=488 nm, Emission=515-545 nm)

Specificity:

Detects human c-Myc in direct ELISAs. Detects human and mouse c-Myc in Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human (rh) L-Myc and rhN-Myc is observed.

View Full Technical Details for Human/Mouse c-Myc Alexa Fluor® 488-conjugated Antibody

Technical Data
Citations / Reviews

Applications

	Recommended Concentration	Sample
Western Blot	Optimal dilution of this antibody should be experimentally determined.
Immunohistochemistry	Optimal dilution of this antibody should be experimentally determined.
Chromatin Immunoprecipitation (ChIP)	Optimal dilution of this antibody should be experimentally determined.
Immunocytochemistry	Optimal dilution of this antibody should be experimentally determined.

Item	Value
Knockout Validated	Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Formulation	Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

=

Mass (in vial)

÷

Desired Reconstitution Concentration

Background: c-Myc

Human c-Myc is a 439 amino acid transcription factor with a bHLH/LZ (basic Helix-Loop-Helix, Leucine Zipper) domain. c-Myc DNA-binding and transcription function is achieved upon heterodimerization with its partner Max. c-Myc is often over-expressed and mutated in hematopoietic tumors. Mutations frequently result in truncations that remove the transactivation region or in the bHLH/LZ domain required for association with Max and DNA. Over the region used as immunogen, human c-Myc is 92% identical to the rat and mouse c-Myc proteins.

Item	Value
Long Name	v-Myc Avian Myelocytomatosis Viral Oncogene Homolog (Avian)
Entrez Gene IDs	4609 (Human); 17869 (Mouse); 24577 (Rat)
Alternate Names	BHLHE39, Class E basic helix-loop-helix protein 39, MYCC, Myc, Myc2, Niard, Nird, Proto-oncogene c-Myc, Transcription factor p64, avian myelocytomatosis viral oncogene homolog, bHLHe39MRTL, c-Myc, cMyc, myc proto-oncogene protein, myc-related translation/localization regulatory factor, v-myc avian myelocytomatosis viral oncogene homolog, v-myc myelocytomatosis viral oncogene homolog (avian)

Customer Reviews

There are currently no reviews for this product. Be the first to review Human/Mouse c-Myc Alexa Fluor® 488-conjugated Antibody and earn rewards!

Have you used Human/Mouse c-Myc Alexa Fluor® 488-conjugated Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review

Product Documents

Product Datasheets

Download Product Datasheets

Human/Mouse c-Myc Alexa Fluor® 488-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # AF3696G

Key Product Details