Human/Mouse BMP-8b Alexa Fluor® 350-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6305U
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Alexa Fluor 350 (Excitation = 346 nm, Emission = 442 nm)
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse BMP-8b
Thr261-His399
Accession # NP_031585
Thr261-His399
Accession # NP_031585
Specificity
Detects mouse BMP-8b in direct ELISAs and human and mouse in Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse (rm) BMP-8a and less than 1% cross‑reactivity with recombinant human (rh) BMP‑3b, r
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Human/Mouse BMP-8b Alexa Fluor® 350-conjugated Antibody
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: BMP-8b
BMP-8b (Bone morphogenetic protein 8b; also OP-3) is a 16 kDa predicted mature and 45 kDa precurser member of the Gbb-60A class of the BMP family of molecules. In mouse, precursors to sperm and oocytes arise from the proximal epiblast around day E7.5 (days 18-20 in human). This lineage commitment is a response to BMP-4 signaling, which is promoted by the repressive action of BMP-8b on the anterior visceral endoderm, a source of BMP-4 antagonism. Mouse BMP-8b preproprecursor is 399 amino acids (aa) in length. It contains a 19 aa signal sequence, a 241 aa prosegment, and a 139 aa mature region. An interchain disulfide bond occurs at Cys363, generating a homodimer. Over aa 261-399, mouse BMP-8b shares 74% aa identity with mouse BMP-8a, and 69% and 93% aa identity with human and rat BMP-8b, respectively.
Long Name
Bone Morphogenetic Protein 8b
Alternate Names
OP-3
Gene Symbol
BMP8B
UniProt
Additional BMP-8b Products
Product Specific Notices for Human/Mouse BMP-8b Alexa Fluor® 350-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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