Western blot shows lysates of RPMI 8226 human multiple myeloma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse BLIMP1/PRDM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3608) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for BLIMP1/PRDM1 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse BLIMP1/PRDM1 Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: RPMI 8226 human multiple myeloma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BLIMP1/PRDM1

BLIMP-1 is a 97 kDa multidomain transcription repressor that has a SET domain in its N-terminal region and four C2H2-type zinc fingers in its C-terminal regions. It promotes terminal differentiation of B cells into plasma cells and also has an important role in primordial germ cell specification. An alternative splice variant that lacks 101 N-terminal amino acid (aa) residues of full-length BLIMP1 has been reported. Over the region used as immunogen, human and mouse BLIMP1 share 89% aa sequence identity.

Long Name

B Lymphocyte Induced Maturation Protein

Alternate Names

PRDM1, ZNFPR1A1

Entrez Gene IDs

639 (Human); 12142 (Mouse); 309871 (Rat)

Gene Symbol

PRDM1

UniProt

AAO45623

Additional BLIMP1/PRDM1 Products

Product Documents for Human/Mouse BLIMP1/PRDM1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Mouse BLIMP1/PRDM1 Antibody

For research use only

Citations
Reviews

Human/Mouse BLIMP1/PRDM1 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF3608

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all BLIMP1/PRDM1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human/Mouse BLIMP1/PRDM1 Antibody

Detection of Human BLIMP1/PRDM1 by Western Blot.