Human/Mouse Bcl-x Antibody
R&D Systems, part of Bio-Techne | Catalog # AF800
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Western Blot, Simple Western, Immunoprecipitation
Cited:
Western Blot, Immunocytochemistry, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Product Specifications
Immunogen
KLH-coupled Bcl-x synthetic peptide
CSAINGNPSWHLADSPAVNGA
CSAINGNPSWHLADSPAVNGA
Specificity
Detects human and mouse Bcl‑x in Western blots.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human/Mouse Bcl-x Antibody
Detection of Human and Mouse Bcl‑x by Western Blot.
Western blot shows lysates of A431 human epithelial carcinoma cell line (5 x 104cells, lane 1 and 2.5 x 104cells, lane 2) and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.3 µg/mL of Rabbit Anti-Human Bcl-x Antigen Affinity-purified Polyclonal Antibody (Catalog # AF800) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Bcl-x at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Immunoprecipitation of Human Bcl-x.
Bcl-x was immunoprecipitated from lysates (7 x 105cells) of A431 human epithelial carcinoma cell line following incubation with 3 µg Rabbit Anti-Human Bcl‑x Antigen Affinity-purified Polyclonal Antibody (Catalog # AF800) for 30‑60 minutes at 4 °C. Bcl-x-antibody complexes were absorbed using Protein A Immunoprecipitin (Life Technologies). Immuno-precipitated Bcl-x was detected by Western blot using 0.3 µg/mL Rabbit Anti-Human Bcl‑x Antigen Affinity-purified Polyclonal Antibody (Catalog # AF800). View our recommended buffer recipes for immunoprecipitation.
Detection of Human and Mouse Bcl-x by Simple WesternTM.
Simple Western lane view shows lysates of A431 human epithelial carcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Bcl‑x at approximately 34 kDa (as indicated) using 3 µg/mL of Rabbit Anti-Human/Mouse Bcl‑x Antigen Affinity-purified Polyclonal Antibody (Catalog # AF800). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human/Mouse Bcl-x Antibody
Application
Recommended Usage
Immunoprecipitation
5 µg/106 cells
Sample: A431 human epithelial carcinoma cell line, see our available Western blot detection antibodies
Sample: A431 human epithelial carcinoma cell line, see our available Western blot detection antibodies
Simple Western
3 µg/mL
Sample: A431 human epithelial carcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line
Sample: A431 human epithelial carcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line
Western Blot
0.3 µg/mL
Sample: A431 human epithelial carcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line
Sample: A431 human epithelial carcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line
Reviewed Applications
Read 1 review rated 5 using AF800 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Bcl-x
Long Name
B Cell Lymphoma/Leukemia x
Alternate Names
BCL2L1, Bclx
Gene Symbol
BCL2L1
Additional Bcl-x Products
Product Documents for Human/Mouse Bcl-x Antibody
Product Specific Notices for Human/Mouse Bcl-x Antibody
For research use only
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