Human/Mouse Bad aa 1‑21 Alexa Fluor® 750-conjugated Antibody

Name: Human/Mouse Bad aa 1‑21 Alexa Fluor® 750-conjugated Antibody
Brand: R&D Systems
SKU: AF819S

R&D Systems, part of Bio-Techne | Catalog # AF819S

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Product Datasheet / COA

Key Product Details

Species Reactivity:

Human, Mouse

Immunogen:

KLH-coupled human Bad synthetic peptide
MFQIPEFEPSEQEDSSSAERGC
Accession # Q92934

Applications:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label:

Alexa Fluor 750 (Excitation=749 nm, Emission=775 nm)

Specificity:

Detects human and mouse Bad in Western blots.

View Full Technical Details for Human/Mouse Bad aa 1‑21 Alexa Fluor® 750-conjugated Antibody

Technical Data
Citations / Reviews

Applications

	Recommended Concentration	Sample
Western Blot	Optimal dilution of this antibody should be experimentally determined.
Immunocytochemistry	Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Formulation	Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

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Background: Bad

Bcl-2 antagonist of cell death (BAD) is an 18 kDa cytoplasmic protein in the Bcl-2 family. It functions as a pro-apoptotic molecule by dimerizing with and inhibiting the anti-apoptotic proteins Bcl-2 and Bcl-xL. Prosurvival signals trigger the phosphorylation of BAD on Ser115, disrupting its interaction with Bcl-2 and Bcl-xL and resulting in protection from apoptosis. Over amino acid 1-21, human BAD shares 81 % aa sequence identity with mouse and rat BAD.

Item	Value
Long Name	Bcl-xL/Bcl-2 Associated Death Promoter
Entrez Gene IDs	572 (Human); 12015 (Mouse); 64639 (Rat)
Alternate Names	BBC2, BBC6, BCL-X/BCL-2 binding protein, BCL2-antagonist of cell death protein, BCL2-associated agonist of cell death, BCL2-binding component 6, BCL2-binding protein, BCL2L8, BCL2L8bcl2-L-8, Bad, Bcl-2-binding component 6, Bcl-2-like protein 8, Bcl-XL/Bcl-2-associated death promoter, Bcl2-L-8, bcl2 antagonist of cell death

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