Detects human MCAM/CD146 in Western blots. In Western blots, less than 5% cross-reactivity with recombinant human (rh) ALCAM, recombinant mouse (rm) MAdCAM-1, and rmOCAM is observed and less than 1% cross-reactivity with rhBCAM, rhEpCAM, rhNCAM-L1, and rhTROP-2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human MCAM/CD146 Biotinylated Antibody

MCAM/CD146 in Human Melanoma.

MCAM/CD146 was detected in immersion fixed paraffin-embedded sections of human melanoma tissue using Goat Anti-Human MCAM/CD146 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF932) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human MCAM/CD146 Biotinylated Antibody

Application

Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human normal breast and human skin cancer tissue

Western Blot

0.1 µg/mL
Sample: Recombinant Human MCAM/CD146

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MCAM/CD146

MCAM, also known as MUC18, is an Ig-superfamily glycoprotein that shows significant homology to several cell adhesion molecules, notably N-CAM. It was identified as an antigen on metastatic melanoma cells and is also expressed on a variety of normal and transformed cell types. MCAM expression is associated with tumor progression and metastasis, and may be involved in embryonic neuronal development.

Long Name

Melanoma Cell Adhesion Molecule

Alternate Names

CD146, L-Gicerin, MUC18

Entrez Gene IDs

4162 (Human); 84004 (Mouse); 78967 (Rat)

Gene Symbol

MCAM

UniProt

AAA20824

Additional MCAM/CD146 Products

Product Documents for Human MCAM/CD146 Biotinylated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human MCAM/CD146 Biotinylated Antibody

For research use only

Citations
Reviews

Human MCAM/CD146 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF932

Key Product Details

Species Reactivity

Validated:

Cited:

Applications