Human LMO2 Antibody

Detection of LMO2 in B-Cell Lymphoma ​via seqIF™ staining on COMET™

LMO2 was detected in immersion fixed paraffin-embedded sections of B-cell lymphoma using Mouse Anti-Human LMO2 Monoclonal Antibody (Catalog # MAB11790) at 0.3 µg/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the nucleus. Protocol available in COMET™ Panel Builder. ​

Immersion fixed paraffin-embedded sections of B-cell lymphoma

LMO2 was detected in immersion fixed paraffin-embedded sections of B-cell lymphoma using Mouse Anti-Human LMO2 Monoclonal Antibody (Catalog # MAB11790) at 0.25 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (VCTS021). Tissue was stained using the HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei of the B-cell Lymphoma. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human LMO2 by Western Blot.

Western Blot shows lysates of K562 human chronic myelogenous leukemia cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.1 µg/ml of Mouse Anti-Human LMO2 Monoclonal Antibody (Catalog # MAB11790) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for LMO2 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.