Human LIF Alexa Fluor™ Plus 555-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # AF-250-NAAFP555

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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Neutralization

Label

Alexa Fluor Plus 555 (Excitation = 558 nm, Emission = 572 nm)

Antibody Source

Polyclonal Goat IgG

View all LIF Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human LIF

Specificity

Detects human LIF in direct ELISAs.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human LIF Alexa Fluor™ Plus 555-conjugated Antibody

Application
Recommended Usage

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Neutralization

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: LIF

LIF (Leukemia inhibitory factor; also  Differentiation-stimulating factor) is a 22 kDa (predicted) glycoprotein, member of  the leukemia inhibitory factor/interleukin-6 (LIF/IL-6) family of cytokines. Natural LIF is heavily glycosylated, showing an apparent molecular weight of 32 kDa to 62 kDa, it is produced by a variety of cells including T cells, monocytes, fibroblasts, osteoblasts and mast cells. LIF induces hematopoietic differentiation in normal and myeloid leukemia cells, neuronal cell differentiation and stimulation of acute-phase protein synthesis in hepatocytes. Human LIF acts through a receptor comprising a 190 kDa LIF-binding alpha-chain and a 130 kDa signal-transducing beta-chain (gp130), which is shared with the other members of the IL-6 family. Human and mouse LIF exhibit 78% aa sequence identity.

Long Name

Leukemia Inhibitory Factor

Alternate Names

D Factor, Emfilermin, HILDA, MLPLI

Entrez Gene IDs

3976 (Human); 16878 (Mouse); 403449 (Canine)

Gene Symbol

LIF

UniProt

P15018

Additional LIF Products

Product Documents

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human LIF Alexa Fluor™ Plus 555-conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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