Detects human LAMP1/CD107a Lumenal Domain in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse LAMP1 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human LAMP-1/CD107a Lumenal Domain Antibody

Detection of Human LAMP‑1/CD107a by Western Blot.

Western blot shows lysates of THP-1 human acute monocytic leukemia cell line and HL-60 human acute promyelocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human LAMP-1/CD107a Lumenal Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4800) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LAMP-1/CD107a at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

LAMP1/CD107a in THP-1 Human Cell Line.

LAMP1/CD107a was detected in immersion fixed THP-1 human acute monocytic leukemia cell line using 10 µg/mL Sheep Anti-Human LAMP1/CD107a Lumenal Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4800) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human LAMP-1/CD107a Lumenal Domain Antibody

Application

Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed THP-1 human acute monocytic leukemia cell line

Western Blot

1 µg/mL
Sample: THP‑1 human acute monocytic leukemia cell line and HL‑60 human acute promyelocytic leukemia cell line

Reviewed Applications

Read 2 reviews rated 5 using AF4800 in the following applications:

Immunocytochemistry (1 Review)
Immunocytochemistry/Immunofluorescence (1 Review)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LAMP-1/CD107a

LAMP1 (Lysosome-associated membrane protein-1; also CD107a) is a 100‑130 kDa member of the LAMP family of glycoproteins. It is expressed in lysosomal and plasma membranes of macrophages, NK and T-cells, and with LAMP2, is essential for the formation of phagolysosomes. On the cell surface, it also presents carbohydrates to selectins. Mature human LAMP1 is a 389 amino acid (aa) type I transmembrane glycoprotein. It contains a 354 aa luminal/extracellular domain (ECD) (aa 28‑381) and a 12 aa cytoplasmic tail (aa 405‑416). The ECD has two large looping regions (aa 28‑193 and 227‑381) plus multiple N- and O-linked glycosylation sites. There is one potential splice variant that shows a 26 aa substitution in the signal sequence. Over aa 28‑380, human LAMP1 shares 64% aa identity with mouse LAMP1.

Long Name

Lysosome-associated Membrane Glycoprotein 1

Alternate Names

CD107a, LAMP1

Entrez Gene IDs

3916 (Human); 16783 (Mouse)

Gene Symbol

LAMP1

UniProt

P11279

Additional LAMP-1/CD107a Products

Product Documents for Human LAMP-1/CD107a Lumenal Domain Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human LAMP-1/CD107a Lumenal Domain Antibody

For research use only

Citations
Reviews

Human LAMP-1/CD107a Lumenal Domain Antibody

R&D Systems, part of Bio-Techne | Catalog # AF4800

Key Product Details

Species Reactivity

Validated:

Cited:

Applications