Human Integrin alphaV/CD51 PE-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB1219P
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry
Label
Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # P2W7
Product Specifications
Immunogen
Ocular melanoma cell line
Specificity
Detects the ectodomain of human Integrin alphaV/CD51.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Integrin alphaV/CD51 PE-conjugated Antibody
Detection of Integrin alphaV/CD51 in HT1080 Human Cell Line by Flow Cytometry.
HT1080 human fibrosarcoma cell line was stained with Mouse Anti-Human Integrin aV/CD51 PE-conjugated Monoclonal Antibody (Catalog # FAB1219P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.Applications for Human Integrin alphaV/CD51 PE-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: HT1080 human fibrosarcoma cell line
Sample: HT1080 human fibrosarcoma cell line
Reviewed Applications
Read 2 reviews rated 5 using FAB1219P in the following applications:
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Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Integrin alpha V/CD51
Integrin alphaV (CD51; also vitronectin receptor subunit alpha) is a 140‑150 kDa member of the integrin alpha-chain family of adhesion molecules. It forms disulfide-linked integral membrane heterodimers with at least five beta-chains, including beta1, 3, 5, 6 and 8. Human alphaV is a 1018 aa type I transmembrane glycoprotein that contains a 962 aa extracellular domain (ECD) (aa 31-992), and a short 32 aa cytoplasmic tail. The ECD contains seven FG (PheAlaGly)-GAP (GlyAlaPro) repeats that form a beta‑propellor domain (aa 46-483). Furin cleavage of the alphaV ECD occurs after Gly889, generating a disulfide-linked heteromeric subunit alphaV chain. alphaV-containing integrins bind multiple ECM molecules, including vitronectin, osteopontin, MMP-2 and TSP. The ECD of human alphaV is 92% aa identical to mouse alphaV ECD.
Alternate Names
CD51, ITGAV, MSK8, Vitronectin Receptor alpha, VNRA
Gene Symbol
ITGAV
Additional Integrin alpha V/CD51 Products
Product Documents for Human Integrin alphaV/CD51 PE-conjugated Antibody
Product Specific Notices for Human Integrin alphaV/CD51 PE-conjugated Antibody
For research use only
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