Human Integrin alphaL/CD11a Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB35951
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Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Flow Cytometry, CyTOF-ready
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # CR38
Product Specifications
Immunogen
Fibronectin purified human monocytes
Specificity
Detects human Integrin alphaL/CD11a in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) Integrin alpha1, alpha2, alpha3, alpha4, alpha5, alpha6, alpha8, alpha9, alpha10, alpha11, alphaM, alphaV, alphaX, rhIntegrin beta3, rhIntegrin alpha6(x1)+ beta4, alpha7(x2)+ beta1, alphaD+ beta2, alphaE+ beta7, recombinant mouse Integrin alpha2b or alphaL is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human Integrin alphaL/CD11a Antibody
Detection of Integrin alphaL/CD11a in Human peripheral blood monocytes by Flow Cytometry.
Human peripheral blood monocytes were stained with Human Integrin aL/CD11a Monoclonal Antibody (Catalog # MAB35951, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B).Applications for Human Integrin alphaL/CD11a Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: Human peripheral blood monocytes
Sample: Human peripheral blood monocytes
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin alpha L/CD11a
Integrin subunit alphaL/CD11a is a 180 kDa type I TM glycoprotein that interacts only with Integrin beta2/CD18 to form LFA-1, a leukocyte adhesion protein which binds endothelial cell ICAM. Human Integrin alphaL contains a 1064 aa extracellular domain (ECD), a 20 aa TM sequence and a 58 aa cytoplasmic domain. The ECD contains seven repeats that form a beta-propeller structure and one inserted vWA domain (I domain) containing a metal ion-dependent adhesion site (MIDAS). Human and mouse Integrin alphaL ECD share 74% aa identity. A second isoform has a 53 aa insert in the ECD.
Alternate Names
CD11a, ITGAL, LFA-1 alpha, Ly-15, Ly-21
Gene Symbol
ITGAL
Additional Integrin alpha L/CD11a Products
Product Documents for Human Integrin alphaL/CD11a Antibody
Product Specific Notices for Human Integrin alphaL/CD11a Antibody
For research use only
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