Human Integrin alpha4/CD49d Antibody
R&D Systems, part of Bio-Techne | Catalog # BBA37
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Adhesion Blockade
Cited:
Neutralization, Flow Cytometry, Immunocytochemistry, Blocking
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 2B4
Product Specifications
Immunogen
U937 human histiocytic lymphoma cell line
Specificity
Detects human Integrin alpha4 subunit (Needham, L.A. et al. (1994) Cell Adhesion and Communication
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Applications for Human Integrin alpha4/CD49d Antibody
Application
Recommended Usage
Adhesion Blockade
The adhesion of U937 human histiocytic lymphoma cells (5 x 104 cells/well) to immobilized recombinant human VCAM-1 (10 μg/mL, 100 µL/well) was maximally inhibited (80 - 100%) by 5 μg/mL of the antibody.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin alpha 4/CD49d
Integrin alpha4, also known as CD49d, is a transmembrane protein that forms heterodimers with beta1 or beta7 integrins. These complexes contribute to the trafficking of many hematopoietic cell types. Integrins alpha4 beta1 (also known as VLA-4) and alpha4 beta7 (LPAM-1) are receptors for fibronectin, VCAM and MAdCAM-1.
Alternate Names
CD49d, ITGA4, VLA-4
Gene Symbol
ITGA4
Additional Integrin alpha 4/CD49d Products
Product Documents for Human Integrin alpha4/CD49d Antibody
Product Specific Notices for Human Integrin alpha4/CD49d Antibody
For research use only
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