Integrin alpha2/CD49b was detected in immersion fixed HT1080 human fibrosarcoma cell line (positive staining) and Daudi human Burkitt's lymphoma cell line (negative staining) using Mouse Anti-Human Integrin alpha2/CD49b Biotinylated Monoclonal Antibody (Catalog # BAM1233) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surface and cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of Integrin alpha2/CD49b in HT1080 cells by Flow Cytometry

HT1080 cells were stained with Mouse Anti-Human Integrin alpha2/CD49b Biotinylated Monoclonal Antibody (Catalog # BAM1233, filled histogram) or isotype control antibody (Catalog # IC003B, open histogram) followed by Streptavidin-Allophycocyanin (Catalog # F0050). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Integrin alpha2/CD49b Biotinylated Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HT1080 human fibrosarcoma cell line

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HT1080 human fibrosarcoma cell line

Immunoprecipitation

Tenchini, M.L. et al. (1993) Cell Adhesion Communication 1:55.

Reviewed Applications

Read 1 review rated 5 using BAM1233 in the following applications:

Immunocytochemistry/Immunofluorescence (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 2/CD49b

Integrin alpha2 is one of twelve integrin family alpha subunits that share the beta1 subunit (1‑3). Integrin alpha2 beta1 is the non-covalent heterodimer of 160 kDa alpha2 (CD49b) and 130 kDa beta1 (CD29) type I transmembrane glycoprotein subunits and is one of six very late antigens on activated T cells, designated VLA2 (3). The alpha2 extracellular domain (ECD) contains an I (inserted) domain which includes the ligand binding site (2, 3). The beta1 ECD contains a vWFA domain, which participates in binding. Each subunit then has a transmembrane sequence and a short cytoplasmic tail. The dimer is folded when it is least active. Divalent cations and intracellular (inside-out) signaling convert it to its most active, extended and open conformation (1, 2). The 1102 amino acid (aa) human alpha2 extracellular domain (ECD) shares 83‑89% aa sequence identity with mouse, rat, canine, bovine and equine alpha2. The I domain-containing beta1 integrins ( alpha1 beta1, alpha2 beta1, alpha10 beta1 and alpha11 beta1) all bind collagens, with alpha2 beta1 preferring collagens I‑III (4, 5). Platelet alpha2 beta1, also called GPIa, cooperates with another adhesion protein, GPVI, to coordinate platelet collagen binding and activation (3, 6, 7). Other alpha2 beta1 ligands include laminin, decorin, E-cadherin, and collagen-like regions of collectin molecules such as C1q (4). Adhesion is synergized by crosstalk with syndecan-1 or HGF R/c-Met, and antagonized by crosstalk with Integrin alpha1 beta1 (8‑10). In addition to expression on selected hematopoietic cells, alpha2 beta1 is present on a wide variety of non-hematopoietic cells (4). Mice deficient in the alpha2 subunit have defects in innate immune responses, wound mast cell infiltration and angiogenesis, and platelet responses to collagen (6, 11, 12). In innate immunity, alpha2 beta1 binding to C1q initiates the complement cascade and costimulates mast cell activation, triggering neutrophil influx (4, 12).

References

Takada, Y. et al. (2007) Genome Biol. 8:215.
Luo, B-H. et al. (2007) Annu. Rev. Immunol. 25:619.
Takada, Y. and M.E. Hemler (1989) J. Cell Biol. 109:397.
Zutter, M.M. and B.T Edelson (2007) Immunobiology 212:343.
McCall-Culbreath, K.D. and M.M. Zutter (2008) Curr. Drug Targets 9:139.
Sarratt, K.L. et al. (2005) Blood 106:1268.
Lecut, C. et al. (2005) Thromb. Haemost. 94:107.
Vuoriluoto, K. et al. (2008) Exp. Cell Res. 314:3369.
McCall-Culbreath, K.D. et al. (2008) Blood 111:3562.
Abair, T.D. et al. (2008) Exp. Cell Res. 314:3593.
Zweers, M. et al. (2006) J. Invest. Dermatol. 127:467.
Edelson, B.T. et al. 2006) Blood 107:143.

Alternate Names

CD49b, ITGA2, VLA-2 alpha

Entrez Gene IDs

3673 (Human); 16398 (Mouse)

Gene Symbol

ITGA2

Additional Integrin alpha 2/CD49b Products

Product Documents for Human Integrin alpha2/CD49b Biotinylated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Integrin alpha2/CD49b Biotinylated Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Integrin alpha2/CD49b Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAM1233

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all Integrin alpha 2/CD49b Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Integrin alpha2/CD49b Biotinylated Antibody

Integrin alpha2/CD49b in HT1080 Human Cell Line.