Detects human IL-23 p19 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human (rh) IL-23 heterodimer is observed, less than 10% cross-reactivity with recombinant mouse (rm) IL-23 heterodimer is observed, and no cross-reactivity with recombinant feline IL-23 p19, recombinant canine (rca) IL-23 p19, and recombinant rat IL-23 p19 is observed.. In Western blots, no cross-reactivity with rcaIL-23 p19 or rmIL-23 p19 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Human IL-23 p19 Antibody

Detection of Human IL‑23 p19 by Western Blot.

Western blot shows lysates of CHO Chinese hamster ovary cell line transfected with human IL-23. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human IL-23 p19 Monoclonal Antibody (Catalog # MAB17161) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL-23 p19 at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Human IL-39 ELISA Standard Curve.

Recombinant Human IL-39 protein was serially diluted 2-fold and captured by Mouse Anti-Human IL-23 p19 Monoclonal Antibody (Catalog # MAB17161) coated on a Clear Polystyrene Microplate (DY990). Mouse Anti-Human EBI3 Monoclonal Antibody (MAB64561) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Applications for Human IL-23 p19 Antibody

Application

Recommended Usage

ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human EBI3 Monoclonal Antibody (Catalog # MAB64561).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Western Blot

1 µg/mL
Sample: CHO Chinese hamster ovary cell line transfected with human IL-23

Reviewed Applications

Read 1 review rated 5 using MAB17161 in the following applications:

ELISA (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-23

Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12 (1‑5). The p19 subunit has homology to the p35 subunit of IL-12, as well as to other single chain cytokines such as IL-6 and IL-11. The p40 subunit is homologous to the extracellular domains of the hematopoietic cytokine receptors. Human and mouse p19 share 70% aa sequence identity. Although p19 is expressed by activated macrophages, dendritic cells, T cells, and endothelial cells, only activated macrophages and dendritic cells express p40 concurrently to produce IL-23. The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta1) and the IL-23-specific receptor subunit (IL-23 R). IL-23 has biological activities that are similar to, but distinct from IL-12. Both IL-12 and IL-23 induce proliferation and IFN-gamma production by human T cells. While IL-12 acts on both naïve and memory human T cells, the effects of IL-23 is restricted to memory T cells. In mouse, IL-23 but not IL-12, has also been shown to induce memory T cells to secret IL-17, a potent proinflammatory cytokine. IL-12 and IL-23 can induce IL-12 production from mouse splenic DC of both the CD8^- and CD8⁺ subtypes, however only IL-23 can act directly on CD8⁺ DC to mediate immunogenic presentation of poorly immunogenic tumor/self peptide.

References

Oppmann, B. et al. (2000) Immunity 13:715.
Lankford, C.S. and D.M. Frucht (2003) J. Leukoc. Biol. 73:49.
Parham, C. et al. (2002) J. Immunol. 168:5699.
Belladonna, M.L. et al. (2002) J. Immunol. 168:5448.
Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910.

Long Name

Interleukin 23

Alternate Names

IL-23 p19/IL-12 p40, IL-23p19/IL-12p40, IL23, SGRF

Entrez Gene IDs

51561 (Human); 83430 (Mouse); 155140 (Rat); 102128555 (Cynomolgus Monkey)

Gene Symbol

IL23A

UniProt

Q9NPF7

Additional IL-23 Products

Product Documents for Human IL-23 p19 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human IL-23 p19 Antibody

For research use only

Citations
Reviews

Human IL-23 p19 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB17161

Key Product Details

Species Reactivity

Validated:

Cited:

Applications