Detects human IL-1 beta/IL-1F2 Propeptide in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-1 alpha, rhIL-1F7, rhIL-1F10, rhIL-18, rhIL-36 alpha, rhIL-36 beta, IL-36 gamma, rhIL-36Ra, recombinant mouse IL-1Ra, or the mature peptides of recombinant IL-1 beta from canine, cotton rat, equine, feline, guinea pig, human, mouse, porcine, rabbit, rat, or rhesus is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human IL-1 beta/IL-1F2 Propeptide Antibody

Detection of Human IL‑1 beta/IL‑1F2 Propeptide by Western Blot.

Western blot shows lysates of THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 80 nM PMA for 48 hours. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IL-1 beta/IL-1F2 Propeptide Monoclonal Antibody (Catalog # MAB6964) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL-1 beta/IL-1F2 Propeptide at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

IL‑1 beta/IL‑1F2 in Human PBMCs.

IL-1 beta/IL-1F2 was detected in immersion fixed human peripheral blood mononuclear cells using Mouse Anti-Human IL-1 beta/IL-1F2 Propeptide Monoclonal Antibody (Catalog # MAB6964) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI(blue). Specific staining was localized to cytoplasmic. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of Human IL‑1 beta/IL‑1F2 Propeptide by Simple Western^TM.

Simple Western lane view shows lysates of THP‑1 human acute monocytic leukemia cell line untreated (-) or treated (+) with PMA and LPS, loaded at 0.2 mg/mL. A specific band was detected for IL‑1 beta/IL‑1F2 Propeptide at approximately 40 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human IL‑1 beta/IL‑1F2 Propeptide Monoclonal Antibody (Catalog # MAB6964). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human IL-1 beta/IL-1F2 Propeptide Antibody

Application

Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs)

Simple Western

10 µg/mL
Sample: THP‑1 human acute monocytic leukemia cell line treated with PMA and LPS

Western Blot

2 µg/mL
Sample: THP‑1 human acute monocytic leukemia cell line treated with PMA

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-1 beta/IL-1F2

IL-1 beta, also known as IL1F2, is a prototypical member of the IL-1 superfamily. It is produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. The biological activity of IL-1 beta is mediated by the heterodimeric receptor complex containing IL-1 R1 (IL-1 RI) and IL-1 R3 (IL-1 RAcP). The IL-1 beta propeptide is cleaved intracellularly by caspase-1/ICE to generate a 17 kDa active cytokine. The propeptide of human IL-1 beta shares approximately 55% aa sequence identity with the propeptides of mouse and rat IL-1 beta.

Long Name

Interleukin 1 beta

Alternate Names

IL-1b, IL-1F2, IL1 beta, IL1B

Entrez Gene IDs

3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)

Gene Symbol

IL1B

UniProt

P01584

Additional IL-1 beta/IL-1F2 Products

Product Documents for Human IL-1 beta/IL-1F2 Propeptide Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human IL-1 beta/IL-1F2 Propeptide Antibody

For research use only

Citations
Reviews

Human IL-1 beta/IL-1F2 Propeptide Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6964

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications