Human IL-1 beta/IL-1F2 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF201

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot, ELISA Detection (Matched Antibody Pair), Immunocytochemistry

Cited:

Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry, Antibody Array Development, Array Development, Bioassay, Electrochemiluminescent Assay, ELISA Detection, ELISA Development, Functional Assay, Luminex Development

Label

Biotin

Antibody Source

Polyclonal Goat IgG

View all IL-1 beta/IL-1F2 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human IL-1 beta/IL-1F2
Ala117-Ser269
Accession # NP_000567

Specificity

Detects human IL-1 beta/IL-1F2 in ELISAs and Western blots. In sandwich immunoassays, less than 4% cross-reactivity with recombinant ratIL‑1 beta is observed and less than 0.1% cross-reactivity with recombinant mouse IL‑1 beta and recombinant porcine IL‑1 beta is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human IL-1 beta/IL-1F2 Biotinylated Antibody

Detection of IL-1 beta/IL-1F2 in THP-1 Human Cell Line.

IL-1 beta/IL-1F2 was detected in immersion fixed THP-1 human acute monocytic leukemia cell line using Goat Anti-Human IL-1 beta/IL-1F2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF201) at 5 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm of THP-1 cells treated with 200nM PMA for 24 hours then 10ug/mL LPS for 24 hours. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
IL-1 beta/IL-1F2 antibody in Human PBMCs by Immunocytochemistry (ICC).

IL‑1 beta/IL‑1F2 in Human PBMCs.

IL-1 beta/IL-1F2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Human IL-1 beta/IL-1F2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF201) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
IL-1 beta/IL-1F2 antibody in Human PBMCs by Immunocytochemistry (ICC).

IL‑1 beta/IL‑1F2 in Human PBMCs.

IL-1 beta/IL-1F2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Human IL-1 beta/IL-1F2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF201) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
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Applications for Human IL-1 beta/IL-1F2 Biotinylated Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs) and THP-1 human acute monocytic leukemia cell line untreated and treated with PMA and LPS

Western Blot

0.1 µg/mL
Sample: Recombinant Human IL-1 beta/IL-1F2 (Catalog # 201-LB)

Human IL-1 beta/IL-1F2 Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Human IL-1 beta/IL-1F2 Antibody (Catalog # MAB601)
  • Capture Reagent: Human IL-1 beta/IL-1F2 Antibody (Catalog # MAB601R)
  • Standard: Recombinant Human IL-1 beta/IL-1F2 Protein (Catalog # 201-LB)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-1 beta/IL-1F2

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2, IL1B), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. The 17 kDa molecular weight mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67%-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta. IL-1 beta functions in a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases.

Long Name

Interleukin 1 beta

Alternate Names

IL-1b, IL-1F2, IL1 beta, IL1B

Entrez Gene IDs

3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)

Gene Symbol

IL1B

UniProt

NP_000567

Additional IL-1 beta/IL-1F2 Products

Product Documents for Human IL-1 beta/IL-1F2 Biotinylated Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human IL-1 beta/IL-1F2 Biotinylated Antibody

For research use only

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