Recombinant Human IL-10 protein was serially diluted 2-fold and captured by Mouse Anti-Human IL-10 Monoclonal Antibody (Catalog # MAB91841) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human IL-10 Monoclonal Antibody (Catalog # MAB2172) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Applications for Human IL-10 Antibody

Application

Recommended Usage

ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human IL‑10 Monoclonal Antibody (Catalog # MAB2172).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human IL-10 DuoSet ELISA Kit (Catalog # DY217B) for convenient development of a sandwich ELISA or the Human IL-10 Quantikine ELISA Kit (Catalog # D1000B) for a complete optimized ELISA.

Reviewed Applications

Read 1 review rated 5 using MAB91841 in the following applications:

ELISA (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-10

Interleukin-10 functions as an anti-inflammatory cytokine by inhibiting the expansion and activation of Th1 cells and Th17 cells and by promoting the development of M2 macrophages and regulatory T cells (Treg). Within a tumor microrenvironment, however, IL-10 can inhibit the expansion of both Treg and myeloid-derived suppressor cells (MDSC). IL-10 exerts protective effects including limiting tissue damage in arthritic inflammation and promoting muscle regeneration after injury, but it also contributes to the persistence of viral infections. IL-10 signals through a receptor complex composed of IL-10 R alpha and IL-10 R beta. IL-10 R beta additionally associates with IL-20 R alpha, IL-22 R alpha 1, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29.

Long Name

Interleukin 10

Alternate Names

CSIF, GVHDS, IL10, IL10A, TGIF

Entrez Gene IDs

3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 102133450 (Cynomolgus Monkey); 100034187 (Equine); 493683 (Feline); 100715618 (Guinea Pig); 2949786 (Viral)

Gene Symbol

IL10

UniProt

P22301

Additional IL-10 Products

Product Documents for Human IL-10 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human IL-10 Antibody

For research use only

Citations
Reviews

Human IL-10 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB91841

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all IL-10 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human IL-10 Antibody

Human IL‑10 ELISA Standard Curve.