Detects human IGF-I R/IGF1R in ELISAs and Western blots. In sandwich immunoassays, less than 1% cross-reactivity with recombinant human (rh) IGF‑I, rhIGF‑II, rhIGFBP-1, rhIGFBP-2, rhIGFBP-3, rhIGFBP-4, rhIGFBP-5, and rhIGFBP-6 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human IGF-I R/IGF1R Biotinylated Antibody

Human IGF-I R ELISA Standard Curve

Recombinant Human IGF-I R/IGF1R (Catalog # 391-GR) was serially diluted and captured by Mouse Anti-Human/Mouse IGF-I R/IGF1R Monoclonal Antibody (Catalog # MAB391) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Mouse IGF-I R/IGF1R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-305-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human IGF-I R/IGF1R Biotinylated Antibody

Application

Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human IGF-I R/IGF1R (Catalog # 391-GR)

Human IGF-I R/IGF1R Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)

Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:

Capture Reagent: Human/Mouse IGF-I R/IGF1R Antibody (Catalog # MAB391)
Standard: Recombinant Human IGF-I R/IGF1R Protein, CF (Catalog # 391-GR)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGF-I R/IGF1R

IGF-I receptor is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha and two beta subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The alpha subunit of IGF-I receptor is extracellular while the beta subunit has an extracellular domain, a transmembrane domain and a cytoplasmic tyrosine kinase domain. The IGF-I receptor is highly expressed in all cell types and tissues. Essentially all of the biological activities of IGF-I and II have been shown to be mediated via IGF-I R.

References

Rechler, M.M. and S.P. Nissley (1990) in Insulin-Like Growth Factors. Sporn, M.B. and A.B. Roberts (eds): Peptide Growth Factors and Their Receptors I, New York: Springer-Verlag, p. 263.

Long Name

Insulin-like Growth Factor I Receptor

Alternate Names

CD221, IGF-1R, IGF-IR, IGF1R, IGFIR

Entrez Gene IDs

3480 (Human)

Gene Symbol

IGF1R

UniProt

P08069

Additional IGF-I R/IGF1R Products

Product Documents for Human IGF-I R/IGF1R Biotinylated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human IGF-I R/IGF1R Biotinylated Antibody

For research use only

Citations
Reviews

Human IGF-I R/IGF1R Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF391

Key Product Details

Species Reactivity

Validated:

Cited:

Applications