Ig Kappa Light Chain Antibody was detected in immersion fixed paraffin-embedded sections of human Appendix using Mouse Anti-Human Ig Kappa Light Chain, Monoclonal Antibody (Catalog # MAB100501) at 1ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 555 Goat anti-Mouse IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.

Detection of Ig Kappa Light Chain in Human Tonsil via seqIF™ staining on COMET™

Ig Kappa Light Chain was detected in immersion fixed paraffin-embedded sections of human Tonsil using Mouse Anti-Human IgKappa, Monoclonal Antibody (Catalog # MAB100501) at 1ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 555 Goat anti-Mouse IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.

Detection of Ig Kappa Light Chain in Human Lymph Node via seqIF™ staining on COMET™

Ig Kappa Light Chain Antibody was detected in immersion fixed paraffin-embedded sections of human lymph node using Mouse Anti-Human Ig Kappa Light Chain, Monoclonal Antibody (Catalog # MAB100501) at 1ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 555 Goat anti-Mouse IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.

Detection of Ig Kappa Light Chain in Human Hodgkin Lymphoma via seqIF™ staining on COMET™

Ig Kappa Light Chain Antibody was detected in immersion fixed paraffin-embedded sections of human Hodgkin Lymphoma using Mouse Anti-Human Ig Kappa Light Chain, Monoclonal Antibody (Catalog # MAB100501) at 1ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 555 Goat anti-Mouse IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.

Detection of Human Ig Kappa Light Chain by Western Blot.

Western blot shows human kappa light chain protein, human lambda light chain protein, human serum, primate serum, mouse serum, and rat serum. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Ig Kappa Light Chain Monoclonal Antibody (Catalog # MAB100501) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Ig Kappa Light Chain at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human Ig Kappa Light Chain by Simple Western^TM.

Simple Western lane view shows lysates of human kappa light chain protein, human lambda light chain protein (negative control), and human serum, loaded at 0.2 mg/mL. A specific band was detected for Ig Kappa Light Chain at approximately 32 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Ig Kappa Light Chain Monoclonal Antibody (Catalog # MAB100501) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Human Ig Kappa Light Chain ELISA Standard Curve.

Human Ig Kappa Light Chain protein was serially diluted 2-fold and captured by Mouse Anti-Human Ig Kappa Light Chain Monoclonal Antibody (Catalog # MAB100501) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Ig Kappa Light Chain Monoclonal Antibody (Catalog # MAB10050) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Detection of Ig Kappa Light Chain in Human Tonsil.

Ig Kappa Light Chain was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human Ig Kappa Light Chain Monoclonal Antibody (Catalog # MAB100501) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

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Applications for Human Ig Kappa Light Chain Antibody

Application

Recommended Usage

COMET

1 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human tonsil, appendix, Hodgkin Lymphoma and lymph node

ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human Ig Kappa Light Chain Monoclonal Antibody (Catalog # MAB10050).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Immunohistochemistry

3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human tonsil

Simple Western

20 µg/mL
Sample: Human kappa light chain protein and Human serum

Western Blot

2 µg/mL
Sample: Human kappa light chain protein and Human serum

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ig Kappa Light Chain

The immunoglobulin light chain is the smaller subunit of an antibody and in humans can be expressed in two types: as a kappa ( kappa) or a lambda ( lambda) chain. Antibodies are produced by B-Cells that are clonal and each expresses only one type of light chain. The light chain class remains fixed for the life of the B lymphocyte. The ratio of kappa and lambda light chains can be used to determine disease status by immunohistochemistry or ELISA based assays.

Long Name

Immunoglobulin Kappa Light Chain

Alternate Names

ig kappa chain c region;Immunoglobulin K;immunoglobulin kappa light chain;Kappa LC

Human Ig Kappa Light Chain Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB100501

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Ig Kappa Light Chain Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Ig Kappa Light Chain Antibody

Detection of Ig Kappa Light Chain in Human Appendix viaseqIF™ staining on COMET™