Human peripheral blood lymphocytes were stained with (A) Mouse Anti-Human HLA-DR PE-conjugated Monoclonal Antibody (Catalog # FAB4869P) or (B) isotype control antibody (Catalog # IC002P) and Mouse anti-Human CD19 APC-conjugated Monoclonal Antibody (Catalog # FAB4867A). View our protocol for Staining Membrane-associated Proteins.

Detection of Human HLA-DR by Flow Cytometry

Costimulatory profile of mature DCs generated with a 3-day protocol. (A) Shown is the expression of specific surface molecules detected on DCs by flow cytometry in a representative patient sample generated with cocktail RP. (B) Comparison of the expression intensity of the costimulatory molecules CD80 and CD86 and the coinhibitory molecules CD273 and CD274 between HCs and AML patients. The mean fluorescence (MFI) of at least three individual experiments of HC and AML patients were compared using the different maturation cocktails C, CP, R, and RP. Image collected and cropped by CiteAb from the following publication (https://translational-medicine.biomedcentral.com/articles/10.1186/1479-…), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human HLA-DR PE-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Human peripheral blood lymphocytes

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: HLA-DR

HLA-DR is a transmembrane human major histocompatibility complex 2 (MHC II) family member and consists of a 34 kDa (alpha) subunit and one of several 28 kDa (beta) subunits. HLA-DR is expressed primarily by B cells and dendritic cells (DC), in which it binds peptides derived from internalized and processed antigenic proteins. It presents these peptides on the cell surface for recognition by the T cell receptor on CD4⁺ T cells. This interaction is central to antigen specificity in the adaptive immune response. HLA-DR alleles, polymorphisms, and aberrant expression are linked to a variety of diseases including autoimmunity and cancer.

Long Name

Major Histocompatibility Complex Class II DR

Alternate Names

HLA-DRA, HLADR, MHC Class II DR

Entrez Gene IDs

3122 (Human)

Gene Symbol

HLA-DRA

UniProt

P01903

Additional HLA-DR Products

Product Documents for Human HLA-DR PE-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human HLA-DR PE-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human HLA-DR PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB4869P

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all HLA-DR Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human HLA-DR PE-conjugated Antibody

Detection of HLA‑DR in Human Blood Lymphocytes by Flow Cytometry.