Human Histone Deacetylase 4/HDAC4 Alexa Fluor™ Plus 405-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB6205AFP405
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry
Label
Alexa Fluor Plus 405 (Excitation = 404 nm, Emission = 455 nm)
Antibody Source
Monoclonal Mouse IgG2A Clone # 713802
Product Specifications
Immunogen
E. coli-derived recombinant human Histone Deacetylase 4/HDAC4
Specificity
Detects human Histone Deacetylase 4/HDAC4 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Applications
Application
Recommended Usage
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: Histone Deacetylase 4/HDAC4
Histone Deacetylase 4 (HDAC4; also HD4) is a founding member of the class IIa subfamily, histone deacetylase family of transcriptional regulators. Although its predicted MW is 119 kDa, it runs anomalously at 140‑150 kDa in SDS‑PAGE which may be due to extensive phosphorylation plus SUMOylation. It has an N-terminal region that interacts with transcription factors and corepressors, and a C-terminal domain that shows deacetylase activity, thus repressing gene transcription. HDAC4 is found in osteoblasts, cardiac and skeletal muscle cells, and neurons. Human HDAC4 is 1084 amino acids (aa) in length. It contains one coiled-coil region (aa 67‑177), a SUMOylation site at Lys559, a histone deacetylation domain (aa 665‑993) and one NES (aa 1051‑1084). Caspase cleavage after Asp289 generates bioactive 97 and 34 kDa fragments. There is one potential splice variant that shows an alternative start site at Met118 coupled to a five aa insertion after Thr431. Over aa 1‑68, human HDAC4 shares 96% aa identity with mouse HDAC4.
Long Name
Histone Deacetylase 4
Alternate Names
HDAC4
Gene Symbol
HDAC4
UniProt
Additional Histone Deacetylase 4/HDAC4 Products
Product Specific Notices
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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