Detects human gp130 in ELISAs and Western blots. In sandwich ELISAs, less than 0.5% cross-reactivity with recombinant mouse gp130, recombinant human (rh) IL‑6, rhIL-11, rhCNTF, and rhOSM is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human gp130 Biotinylated Antibody

Human gp130 ELISA Standard Curve

Recombinant Human gp130 (Catalog # 228-GP) was serially diluted and captured by Mouse Anti-Human gp130 Monoclonal Antibody (Catalog # MAB628) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human gp130 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-228-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human gp130 Biotinylated Antibody

Application

Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human gp130 (Catalog # 228-GP)

Human gp130 Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)

Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:

Capture Reagent: Human gp130 Antibody (Catalog # MAB628)
Standard: Recombinant Human gp130 Protein (Catalog # 228-GP)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using BAF228 in the following applications:

ELISA (1 Review)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: gp130

Gp130, the common signal transducing receptor component shared by the functional receptor complexes of the IL-6 family of cytokines, belongs to the class I cytokine receptor family. Binding of IL-6 (IL-11) to either the membrane-anchored or soluble IL-6 R (IL-11 R) initiates the association of IL-6 R (IL-11 R) with gp130 which then undergoes homo-dimerization and signal transduction. With other IL-6 family cytokines, such as LIF and OSM, signal transduction is triggered by the hetero-dimerization of gp130 and LIF R or OSM R.

Gp130 is expressed in all organs examined. Soluble gp130, which apparently arises either from proteolytic cleavage of the membrane-bound receptor or from alternative splicing, has been detected in human serum. At the present time, the in vivo functions of soluble gp130 are not clearly understood. In in vitro experiments, natural or recombinant soluble gp130 has been shown to have inhibitory effects on OSM and CNTF activities.

References

Narazaki, M. et al. (1993) Blood 82:1120.
Taga, T. and T. Kishimoto (1997) Annu. Rev. Immunol. 15:797.

Long Name

Glycoprotein 130

Alternate Names

CD130, IL6ST

Entrez Gene IDs

3572 (Human); 16195 (Mouse); 25205 (Rat); 101926138 (Cynomolgus Monkey)

Gene Symbol

IL6ST

UniProt

P40189

Additional gp130 Products

Product Documents for Human gp130 Biotinylated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human gp130 Biotinylated Antibody

For research use only

Citations
Reviews

Human gp130 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF228

Key Product Details

Species Reactivity

Validated:

Cited:

Applications