Human GITR/TNFRSF18 PE-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB689P
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Flow Cytometry
Cited:
Flow Cytometry
Label
Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 110416
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human GITR/TNFRSF18
Gln26-Glu161 (Thr43Ala)
Accession # Q9Y5U5
Gln26-Glu161 (Thr43Ala)
Accession # Q9Y5U5
Specificity
Detects human GITR in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) 4‑1BB, recombinant mouse (rm) 4‑1BB, rhCD27, rmCD27, rhCD30, rmCD30, rhCD40, rmCD40, rhDR3, rhDR6, rhEDAR, rmEDAR, rhFas, rmFAS, rmGITR, rhHVEM, rhLymphotoxin R beta, rmLymphotoxin R beta, rhNGF R, rhOPG, rmOPG, rhRANK, rmRANK, rhTAJ, rhTNF RI, rmTNF RI, rhTNF RII, or rmTNF RII.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human GITR/TNFRSF18 PE-conjugated Antibody
Detection of GITR/TNFRSF18 in Human PBMCs by Flow Cytometry.
Human peripheral blood mononuclear cells (PBMCs) treated with 1 µg/mL PHA for 24 hours were stained with Mouse Anti-Human GITR/TNFRSF18 PE-conjugated Mono-clonal Antibody (Catalog # FAB689P, filled histogram) or isotype control antibody (IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of GITR/TNFRSF18 in RPMI8226 (positive cells) vs. Jurkat (negative cells) by Flow Cytometry
RPMI8226 cells (filled histogram) vs. Jurkat cells (open histogram) were stained with Mouse Anti-Human GITR/TNFRSF18 PE-conjugated Monoclonal Antibody (Catalog # FAB689P). View our protocol for Staining Membrane-associated Proteins.Applications for Human GITR/TNFRSF18 PE-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: - Human peripheral blood mononuclear cells (PBMCs) treated with PHA
- RPMI8226 cells
Sample: - Human peripheral blood mononuclear cells (PBMCs) treated with PHA
- RPMI8226 cells
Reviewed Applications
Read 1 review rated 3 using FAB689P in the following applications:
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: GITR/TNFRSF18
GITR (glucocorticoid-induced tumor necrosis factor receptor, also named AITR, activation-inducible TNF R family member), is a 228 amino acid (aa) type I transmembrane protein belonging to the TNF R family and has been designated TNFRSF18. The GITR cytoplasmic domain has striking homology with the cytoplasmic domain of 4-1BB and CD27. Human GITR shares 55% homology with murine GITR. GITR is expressed at low levels in peripheral blood T cells, bone marrow, thymus, spleen, and lymph nodes. In contrast to mouse GITR, expression of human GITR is not induced by treatment with dexamethasone, but is up-regulated by antigen stimulation or by treatment with anti-CD3 plus anti-CD28, or PMA plus ionomycin. Human GITR ligand was identified from human umbilical vein endothelial cells and is a 177 aa polypeptide belonging to the TNF superfamily (TNFSF18). Ligation of GITR can activate NF-kappa B through TRAF2, and protect T cells from TCR activation-induced cell death. It has been proposed that GITR ligand and GITR may modulate T lymphocyte functions.
References
- Nocentini, G. et al. (1997) Proc. Natl. Acad. Sci. USA 94:6216.
- Kwon, B. et al. (1999) J. Biol. Chem. 274:6056.
- Gurney, A.L. et al. (1999) Current Biology 9:215.
- Kwon, B. et al. (1999) Current Opinion in Immunology 11:340.
Long Name
Glucocorticoid Induced TNF Receptor Family Related Gene
Alternate Names
AITR, CD357, TNFRSF18
Gene Symbol
TNFRSF18
UniProt
Additional GITR/TNFRSF18 Products
Product Documents for Human GITR/TNFRSF18 PE-conjugated Antibody
Product Specific Notices for Human GITR/TNFRSF18 PE-conjugated Antibody
For research use only
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