Detects human Galectin-9 in Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human (rh) Galectin-3 and rhGalectin-4 is observed and less than 2% cross-reactivity with rhGalectin-1, rhGalectin-2, rhGalectin-7, and rhGalectin-8 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Galectin-9 Biotinylated Antibody

Galectin-9 in U937 Human Cell Line.

Galectin-9 was detected in immersion fixed U937 human histiocytic lymphoma cell line using Goat Anti-Human Galectin-9 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2045) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human Galectin-9 Biotinylated Antibody

Application

Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed U937 human histiocytic lymphoma cell line

Western Blot

0.1 µg/mL
Sample: Recombinant Human Galectin-9

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Galectin-9

Galectin-9 (also known as Ecalectin and UAT) is a 40 kDa beta-galactoside binding lectin that belongs to a tandem repeat group of Galectins. Activated cells such as T cells, PMNs, astrocytes, fibroblasts and endothelial cells can express Galectin-9, but its release requires select MMPs. Human to mouse, there is 69% aa sequence identity for Galectin-9.

Alternate Names

Ecalectin, GAL9, Galectin9, LGALS9

Entrez Gene IDs

3965 (Human); 16859 (Mouse)

Gene Symbol

LGALS9

Additional Galectin-9 Products

Product Documents for Human Galectin-9 Biotinylated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Galectin-9 Biotinylated Antibody

For research use only

Citations
Reviews

Human Galectin-9 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF2045

Key Product Details

Species Reactivity

Validated:

Cited:

Applications