Human FRAT2 Alexa Fluor® 488-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7980G
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry
Label
Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human FRAT2
Ser31-Leu233 (Ala83Thr)
Accession # O75474
Ser31-Leu233 (Ala83Thr)
Accession # O75474
Specificity
Detects human FRAT2 in direct ELISAs.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Human FRAT2 Alexa Fluor® 488-conjugated Antibody
Application
Recommended Usage
Immunocytochemistry
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: FRAT2
FRAT2 (Frequently Rearranged in Advanced T-cell lymphomas-2; also GSK-3-binding protein FRAT2) is an intracellular member of the GSK-3-binding protein family. Although its predicted MW is 28 kDa, due to its highly acidic nature, it runs anomalously at 35 kDa in SDS-PAGE. It is widely expressed, and plays a key role in the regulation of the Wnt signaling pathway. Typically, in the absence of stimulation, GSK-3 beta and beta-catenin associate with Axin, where GSK-3 beta phosphorylates beta-catenin, leading to its turnover. Upon Wnt stimulation, GSK-3 beta phosphorylation of beta-catenin is decoupled, resulting in beta-catenin mediated gene activation. FRAT2 has the same effect as Wnt stimulation on GSK-3 beta activity, serving as a non-Wnt activator of beta-catenin. It does so by promoting the dissociation of GSK-3 beta from axin. It also interacts with Diversin, a participant in JNK signaling. This suggests that FRAT2 plays a role in both Wnt/ beta-catenin and PCP signaling pathways. Human FRAT2 is 233 amino acids (aa) in length. It contains an N-terminal acidic domain (aa 1-54), a Pro-rich segment (aa 60-107) and a GSK-3 beta binding region (aa 171-196). Over aa 31‑233, human FRAT2 shares 73% aa sequence identity with both mouse FRAT2 and human FRAT1.
Long Name
Frequently Rearranged in Advanced T-cell Lymphomas 2
Alternate Names
frequently rearranged in advanced T-cell lymphomas 2FRAT-2, GSK-3 binding protein FRAT2, GSK-3-binding protein FRAT2, MGC10562
Gene Symbol
FRAT2
UniProt
Additional FRAT2 Products
Product Specific Notices for Human FRAT2 Alexa Fluor® 488-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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