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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Alexa Fluor 700 (Excitation = 675-700 nm, Emission = 723 nm)

Antibody Source

Polyclonal Sheep IgG

Product Specifications

Immunogen

E. coli-derived recombinant human FLI1
Ser13-Pro112
Accession # Q01543

Specificity

Detects human FLI1 in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Human FLI1 Alexa Fluor® 700-conjugated Antibody

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: FLI1

FLI1 (Friend leukemia virus integration site 1; also ERGB) is a member of the ETS family of transcription factors. Although its predicted MW is 51 kDa. FLI1 is expressed by megakaryocytes, macrophages, B cells and embryonic endothelial cells. It is a transcriptional activator that regulates the genes for Tie-2, GpIIb, MPL and TGF-beta RII. Human FLI1 is 452 amino acids (aa) in length. It contains one PNT domain that is involved in heterotypic interactions (aa 112-198) and an ETS DNA‑binding domain (aa 281-361). There are two potential splice variants. One shows an alternative start site at Met34, while another contains a 10 aa substitution for aa 1‑76. There are a series of naturally occurring 62-68 kDa chimeric proteins (EWS/FLI1) in tumors that result from a chromosomal translocation involving the EWS and FLI1 genes. One creates a 499 aa chimera that includes aa 1-265 of EWS and 220-452 of FLI1. Over aa 13-112, human FLI1 shares 98% aa identity with mouse FLI1.

Long Name

Friend Leukemia Integration 1

Alternate Names

ERGB, SIC-1

Entrez Gene IDs

2313 (Human); 14247 (Mouse); 315532 (Rat)

Gene Symbol

FLI1

UniProt

Additional FLI1 Products

Product Documents for Human FLI1 Alexa Fluor® 700-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human FLI1 Alexa Fluor® 700-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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