Human Fibroblast Activation Protein  alpha/FAP Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB3715

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Xenograft

Applications

Validated:

ELISA Capture (Matched Antibody Pair), Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Flow Cytometry, Immunocytochemistry, Cell Capture, Cell Culture, FACS, Functional Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 427819

View all Fibroblast Activation Protein alpha/FAP Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Fibroblast Activation Protein alpha/FAP
Leu26-Asp760
Accession # Q12884

Specificity

Detects human Fibroblast Activation Protein alpha/FAP in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human DPP6 and DPP4 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Fibroblast Activation Protein  alpha/FAP Antibody

Detection of Fibroblast Activation Protein a/FAP antibody in WI-38 Human Cell Line antibody by Flow Cytometry.

Detection of Fibroblast Activation Protein alpha/FAP in WI‑38 Human Cell Line by Flow Cytometry.

WI-38 human lung fibroblast cell line was stained with Mouse Anti-Human Fibroblast Activation Protein a/FAP Monoclonal Antibody (Catalog # MAB3715, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Detection of Fibroblast Activation Protein  alpha/FAP in U-87 MG cells by Flow Cytometry.

U-87 MG cells were stained with Mouse Anti-Human Fibroblast Activation Protein alpha/FAP Monoclonal Antibody (Catalog # MAB3715, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Fluorescein-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0103B). View our protocol for Staining Membrane-associated Proteins.
Detection of Fibroblast Activation Protein alpha /FAP by Immunohistochemistry

Detection of Fibroblast Activation Protein alpha /FAP by Immunohistochemistry

The expression of FAP and EGFR and clinical outcome in 132 cases of esophageal cancer. (A) IHC for FAP and EGFR are shown at low and high magnification (40 × and 200 ×). Scale bars: 500 µm (40 ×), 100 µm (200 ×). The expression of FAP and EGFR is different between intra-tumoral and peri-tumoral tissue. (B) Representative example of a low- and high-FAP and EGFR case. Scale bars: 200 µm (100 ×). The FAP score of 4 + or more were defined as high, that of 3 + or less were low; the EGFR expression level of 2 + or 3 + were defined as high, that of 0 or 1 + were low. (C) Survival analysis showed that FAP high patients had significantly worse OS than those with low FAP (P < 0.001, log-rank test; *P < 0.05). (D) Patients with high EGFR tended to show poorer OS than those with low EGFR (P = 0.074, log-rank test). (E) Survival curve of three groups divided by combination of EGFR and FAP scores (double negative, single positive, double positive), double positive group had worse survival than others (P = 0.005: vs. double negative, P = 0.089: vs. single positive, log-rank test; *P < 0.05). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36418422), licensed under a CC-BY license. Not internally tested by R&D Systems.
View 3 more images

Applications for Human Fibroblast Activation Protein  alpha/FAP Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: WI‑38 human lung fibroblast cell line  and U‑87 MG human glioblastoma/astrocytoma cell line

Human Fibroblast Activation Protein alpha/FAP Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human Fibroblast Activation Protein  alpha/FAP Biotinylated Antibody (Catalog # BAF3715)
  • Standard: Recombinant Human FAP Protein, CF (Catalog # 3715-SE)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 5 using MAB3715 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fibroblast Activation Protein alpha/FAP

FAP (also known as Seprase) is a 97 kDa Type II transmembrane serine protease that is structurally related to Dipeptidyl Peptidase IV (DPPIV) (1). FAP has substrate specificity similar to DPPIV, which is specific for N-terminal Xaa-Pro sequences, but FAP is also an endopeptidase able to degrade gelatin and Type I Collagen (2). The enzymatically active form of FAP is a dimer that migrates at ~170 kDa. It is associated with multiple integral membrane proteins such as Integrin  alpha3 beta1, UPA and DPPIV (3,4). FAP has a restricted tissue distribution. It is occasionally detected in fibroblasts and pancreatic islet cells, but is highly expressed on reactive stromal fibroblasts in epithelial cancers, in granulation tissue during wound healing, and in bone and soft tissue sarcomas (4-6). Because of its expression patterns and enzymatic activities, FAP is believed to play roles in tumor invasion, tissue remodeling, and wound repair. The 760 amino acid (aa) human FAP contains a 735 aa extracellular domain that is glycosylated and necessary for activity (4). It shares 90% aa identity with mouse and rat FAP. A reported 672 aa splicing variant diverges prior to the active site charge relay residues at the C-terminus.

References

  1. Scanlan, M.J. et al. (1994) Proc. Natl. Acad. Sci. USA 91:5657.
  2. Park, J.E. et al. (1999) J. Biol. Chem. 274:36505.
  3. Pineiro-Sanchez, M.L. et al. (1997) J. Biol. Chem. 272:7595.
  4. O'Brien, P. and B.F. O'Connor (2008) Biochim. Biophys. Acta 1784:1130.
  5. Garin-Chesa, P. et al. (1990) Proc. Natl. Acad. Sci. USA 87:7235.
  6. Rettig, W.J. et al. (1988) Proc. Natl. Acad. Sci. USA 85:3110.

Alternate Names

FAP, Seprase

Entrez Gene IDs

2191 (Human); 14089 (Mouse); 102134935 (Cynomolgus Monkey)

Gene Symbol

FAP

UniProt

Q12884

Additional Fibroblast Activation Protein alpha/FAP Products

Product Documents for Human Fibroblast Activation Protein  alpha/FAP Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Fibroblast Activation Protein  alpha/FAP Antibody

For research use only

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