Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Fatty Acid Synthase/FASN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5927) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Fatty Acid Synthase/FASN at approximately 220 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Western Blot Shows Human Fatty Acid Synthase/FASN Specificity by Using Knockout Cell Line.

Western blot shows lysates of HEK293 human embryonic kidney parental cell line and Fatty Acid Synthase/FASN knockout HEK293 cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Fatty Acid Synthase/FASN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5927) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Fatty Acid Synthase/FASN at approximately 275 kDa (as indicated) in the parental HEK293 cell line, but is not detectable in knockout HEK293 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human Fatty Acid Synthase/FASN by Simple Western^TM.

Simple Western shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.5 mg/ml. A specific band was detected for Fatty Acid Synthase/FASN at approximately 213 kDa (as indicated) using 20 µg/mL of Goat Anti-Human Fatty Acid Synthase/FASN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5927). This experiment was conducted under reducing conditions and using the 66-440kDa separation system.

Applications for Human Fatty Acid Synthase/FASN Antibody

Application

Recommended Usage

Knockout Validated

Fatty Acid Synthase/FASN is specifically detected in HEK293 human embryonic kidney parental cell line but is not detectable in Fatty Acid Synthase/FASN knockout HEK293 cell line.

Simple Western

20 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line

Western Blot

1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fatty Acid Synthase/FASN

FASN (Fatty Acid SyNthase; also FAS) is a cytosolic member of the short chain dehydrogenase/reductase family 27X. It is expressed in neurons, skeletal muscle, adipocytes and hepatocytes and both catalyzes the formation of palmitic acid from acetylCoA and malonylCoA and likely mediates the transfer of fatty acids to peptides. Human FASN is 2511 amino acids (aa) in length. It contains multiple domains, including a beta‑ketoacyl synthase domain (aa 1‑414), an acyl and malonyl transferase domain (aa 429‑817), an enoyl reductase domain (aa 1563‑1863), a beta‑ketoacyl reductase domain (aa 1864‑2118) and a thioesterase domain (aa 2207‑2511). FASN exists as an antiparallel homodimer. There is one theoretical alternative start site at Met2073. Over aa 10‑212, human FASN shares 92% aa identity with mouse FASN.

Alternate Names

FASN, OA-519, SDR27X1

Entrez Gene IDs

2194 (Human); 14104 (Mouse)

Gene Symbol

FASN

UniProt

P49327 (Human)

Additional Fatty Acid Synthase/FASN Products

Product Documents for Human Fatty Acid Synthase/FASN Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Fatty Acid Synthase/FASN Antibody

For research use only

Citations
Reviews

Human Fatty Acid Synthase/FASN Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5927

Key Product Details

Validated by

Species Reactivity

Applications

Label

Antibody Source

View all Fatty Acid Synthase/FASN Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Fatty Acid Synthase/FASN Antibody

Detection of Human Fatty Acid Synthase/FASN by Western Blot.