Human F-box protein 15/FBXO15 Alexa Fluor™ Plus 647-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB6035AFP647
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Intracellular Staining by Flow Cytometry, Immunocytochemistry, CyTOF-ready
Label
Alexa Fluor Plus 647 (Excitation = 658 nm, Emission = 675 nm)
Antibody Source
Monoclonal Mouse IgG2A Clone # 612834
Product Specifications
Immunogen
E. coli-derived recombinant human F‑box protein 15/FBXO15 isoform 2
Specificity
Detects human F‑box protein 15/FBXO15 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse F-box protein 15 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Applications
Application
Recommended Usage
CyTOF-ready
Optimal dilution of this antibody should be experimentally determined.
Immunocytochemistry
Optimal dilution of this antibody should be experimentally determined.
Intracellular Staining by Flow Cytometry
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: F-box protein 15/FBXO15
FBXO15 (F-box only protein 15; also FBX15) is a 50‑60 kDa member of the F-box family, O-subfamily of molecules that is most closely related to FBXO8 and O11. It is found in pluripotent stem cells and testis tissue, and apparently serves as a substrate recognition component of the SCF-type E3 ubiquitin ligase. Human FBXO15 (isoform 2 of accession Q8NCQ5) is 434 amino acids (aa) in length. It contains one F-box (aa 1‑41) that likely serves as an interaction domain for SKP1. There are two potential isoforms, one that contains an alternative start site 76 aa upstream of the standard site (isoform 1 of accession Q8NCQ5), and a second that shows complex splicing that includes a 66 aa insertion after Asn35, a Ser substitution for aa 186‑256, and a 106 aa substitution for aa 304‑346. Over aa 298‑434, human FBXO15 shares 53% aa identity with mouse FBXO15.
Alternate Names
Fbox protein 15, FBX15, FBXO15
Gene Symbol
FBXO15
UniProt
Additional F-box protein 15/FBXO15 Products
Product Specific Notices
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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