PBMC monocytes were stained with Mouse Anti-Human/Equine CD11b/Integrin alpha M Monoclonal Antibody (Catalog # MAB16991, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human/Equine CD11b/Integrin alpha M Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: PBMC monocytes

Reviewed Applications

Read 1 review rated 4 using MAB16991 in the following applications:

Immunohistochemistry (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD11b/Integrin alpha M

The Integrin alphaM subunit (CD11b), associates with the Integrin beta2 subunit (CD18) to form the non-covalent heterodimeric Integrin CD11b/CD18, also known as Mac-1 and CR-3. Upon activation, CD11b/CD18 is expressed on granulocytes, monocytes, a subset of NK cells and activated lymphocytes. Integrin CD11b/CD18 functions as a receptor for complement fragment iC3b, ICAM-1 (CD54), ICAM-2 (CD102) and fibrinogen to mediate phagocyte adhesion, migration and ingestion of complement-opsonized particles (1‑3).

References

Springer, T.A. et al. (1978) Eur. J. Immunol. 8:539.
Springer, T.A. et al. (1979) Eur. J. Immunol. 9:301.
Springer, T.A. et al. (1982) Immunol. Rev. 68:171.

Alternate Names

CD11b, Integrin alpha M, ITGAM

Entrez Gene IDs

3684 (Human); 16409 (Mouse)

Gene Symbol

ITGAM

UniProt

NP_001139280

Additional CD11b/Integrin alpha M Products

Product Documents for Human/Equine CD11b/Integrin alpha M Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Equine CD11b/Integrin alpha M Antibody

For research use only

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Human/Equine CD11b/Integrin alpha M Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB16991

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all CD11b/Integrin alpha M Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human/Equine CD11b/Integrin alpha M Antibody

Detection of CD11b/Integrin alpha M in PBMC monocytes by Flow Cytometry.