DPPA2 was detected in immersion fixed BG01V human embryonic stem cells using Sheep Anti-Human DPPA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7086) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human DPPA2 Antibody

Application

Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DPPA2

DPPA2 (Developmental pluripotency associated 2; also ESCA, CT100 and PESCRG1) is a 38-39 kDa member of both the DPPA and cancer-testis family of proteins. It is found in pluripotent stem cells in a pattern that parallels that of Oct4, and likely contributes to formation of the ICM, plus the maintainence of cells in an undifferentiated state. DPPA2 is also found in spermatocytes and select tumor types. Human DPPA2 is 298 amino acids (aa) in length. It contains one DNA-binding SAP domain that is involved in chromosomal organization (aa 92-126). One potential isoform variant has been reported that shows a deletion of aa 61-71. Over aa 2‑174, human DPPA2 shares only 36% aa identity with mouse DPPA2.

Long Name

Developmental Pluripotency Associated Protein 2

Alternate Names

CT100, ECSA, PESCRG1

Entrez Gene IDs

151871 (Human); 100043167 (Mouse); 685364 (Rat)

Gene Symbol

DPPA2

UniProt

Q7Z7J5 (Human)

Additional DPPA2 Products

Product Documents for Human DPPA2 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human DPPA2 Antibody

For research use only

Citations
Reviews

Human DPPA2 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF7086

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all DPPA2 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human DPPA2 Antibody

DPPA2 in BG01V Human Embryonic Stem Cells.