Human DLEC/CLEC4C/BDCA-2 Alexa Fluor™ Plus 594-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # AF1376AFP594

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AF1376AFP594-100UG
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Flow Cytometry, CyTOF-ready

Label

Alexa Fluor Plus 594 (Excitation = 590 nm, Emission = 618 nm)

Antibody Source

Polyclonal Goat IgG

View all DLEC/CLEC4C/BDCA-2 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human DLEC/CLEC4C/BDCA‑2

Specificity

Detects human DLEC/CLEC4C/BDCA‑2 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications

Application
Recommended Usage

CyTOF-ready

Optimal dilution of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: DLEC/CLEC4C/BDCA-2

Dendritic cell lectin (DLEC), also known as BDCA-2, CD303, HECL, and CLEC4C/CLECSF11/CLECSF7, is a 38 kDa type II transmembrane protein in the C-type lectin family (1). Mature human DLEC consists of a 21 amino acid (aa) cytoplasmic domain, a 23 aa transmembrane segment, and a 169 aa extracellular domain (ECD) that contains a juxtamembrane neck region and one carbohydrate recognition domain (CRD) (2, 3). Alternate splicing may generate multiple isoforms that lack the transmembrane segment and/or portions of the cytoplasmic, neck, and CRD regions (2-4). An ortholog of human DLEC has not been described in mouse or rat. DLEC expression is restricted to plasmacytoid dendritic cells (pDC) and is downregulated during their maturation (2, 3, 5). pDC play a role in the innate immune response by producing IFN-alpha / beta following exposure to TLR7 and TLR9 agonists such as microbial CpG DNA (3, 5-8). Antibody ligation of DLEC on pDC attenuates the CpG-stimulated production of interferons as well as a Th1 biased response (3, 5-9). DLEC interactions with HIV-1 gp120 and hepatitis B virus soluble antigen may therefore limit the pDC antiviral response (10, 11). Similar to other C-type lectins, DLEC can mediate antigen uptake for MHC loading and presentation to T cells (3, 12). Crosslinking of DLEC on CpG-stimulated pDC inhibits pDC maturation and induces tyrosine phosphorylation on multiple proteins involved in B cell receptor signaling and endocytosis (5, 7, 8). These functions require the association of DLEC with the ITAM-containing Fc epsilon RI gamma chain (7, 8).

References

  1. Kanazawa, N. et al. (2004) Immunobiology 209:179.
  2. Arce, I. et al. (2001) Eur. J. Immunol. 31:2733.
  3. Dzionek, A. et al. (2001) J. Exp. Med. 194:1823.
  4. Fernandes, M.J. et al. (2000) Genomics 69:263.
  5. Wu, P. et al. (2008) Clin. Immunol. 129:40.
  6. Fanning, S.L. et al. (2006) J. Immunol. 177:5829.
  7. Rock, J. et al. (2007) Eur. J. Immunol. 37:3564.
  8. Cao, W. et al. (2007) PloS Biol. 5:e248.
  9. Riboldi, E. et al. (2009) Immunobiology 214:868.
  10. Martinelli, E. et al. (2007) Proc. Natl. Acad. Sci. USA 104:3396.
  11. Xu, Y. et al. (2009) Mol. Immunol. 46:2640.
  12. Jaehn, P.S. et al. (2008) Eur. J. Immunol. 38:1822.

Long Name

Dendritic Cell Lectin

Alternate Names

BDCA-2, BDCA2, CD303, CLEC4C, CLECSF11, CLECSF7

Entrez Gene IDs

170482 (Human)

Gene Symbol

CLEC4C

UniProt

Q8WTT0

Additional DLEC/CLEC4C/BDCA-2 Products

Product Documents

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Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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