Detects human Dkk-1 in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant human (rh) Dkk-4, rhKremen-1, rhKremen-2 or rhLRP-6 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Human Dkk-1 Antibody

Dkk‑1 in Human Colon.

Dkk-1 was detected in immersion fixed paraffin-embedded sections of human colon using Human Dkk-1 Monoclonal Antibody (Catalog # MAB10962) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Human Dkk-1 ELISA Standard Curve

Recombinant Human Dkk-1 (Catalog # 5439-DK) was serially diluted and captured by Mouse Anti-Human Dkk-1 Monoclonal Antibody (Catalog # MAB10962) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Mouse Dkk-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1096) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human Dkk-1 Antibody

Application

Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human colon

Human Dkk-1 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)

Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:

Detection Reagent: Human Dkk-1 Biotinylated Antibody (Catalog # BAF1096)
Standard: Recombinant Human Dkk-1 Protein (Catalog # 5439-DK)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 5 using MAB10962 in the following applications:

ELISA (1 Review)
Immunohistochemistry (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Dkk-1

Dkk-1 (Dickkopf related protein 1) is a secreted antagonist of Wnt/beta-Catenin signaling. It interacts with LRP-6, and internalized complexes of Dkk-1/LRP-6/Kremen 2 can downregulate Wnt signaling. The balance between Wnt signaling and Dkk-1 inhibition is critical for bone formation and homeostasis. In addition, Dkk-1 can act as either a tumor suppressor or promoter of metastasis.

Long Name

Dickkopf-1

Alternate Names

Dkk1

Entrez Gene IDs

22943 (Human); 13380 (Mouse); 102123735 (Cynomolgus Monkey)

Gene Symbol

DKK1

UniProt

O94907

Additional Dkk-1 Products

Product Documents for Human Dkk-1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Dkk-1 Antibody

For research use only

Citations
Reviews

Human Dkk-1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB10962

Key Product Details

Species Reactivity

Validated:

Cited:

Applications