Desmin was detected in immersion fixed paraffin-embedded sections of human Skeletal Muscle using Rabbit Anti-Human Monoclonal Antibody (Catalog # MAB11654) at 0.1ug/mL at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cell membrane. Protocol available in COMET™ Panel Builder.

Detection of Human, Mouse and Rat Desmin by Western Blot.

Western Blot shows lysates of RD human rhabdomyosarcoma cell line, C2C12 mouse myoblast cell line and rat heart tissue. PVDF membrane was probed with 1 µg/ml of Rabbit Anti-Human Desmin Monoclonal Antibody (Catalog # MAB11654) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Desmin at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of Desmin in Human Heart.

Desmin was detected in immersion fixed paraffin-embedded sections of human heart using Rabbit Anti-Human Desmin Monoclonal Antibody (Catalog # MAB11654) at 3 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003) or the HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of cardiomyocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

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Applications for Human Desmin Antibody

Application

Recommended Usage

Immunohistochemistry

1-10 µg/mL
Sample: Immersion fixed paraffin embedded sections of human heart and human skeletal muscle

Multiplex Immunofluorescence

0.1 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human Skeletal Muscle

Western Blot

1 µg/mL
Sample: RD human rhabdomyosarcoma cell line, C2C12 mouse myoblsat cell line and rat heart tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Desmin

Desmin is a 53 kDa, muscle-specific molecule that belongs to the intermediate filament superfamily of cytoskeletal proteins. Cytoplasmic Desmin homopolymers form stable filamentous networks that stabilize lateral alignments of myofibrils. Human Desmin is 470 amino acid peptide. It contains an N-terminal head segment (aa 1‑84), multiple coiled regions (aa 109‑412) and a tail region (aa 413‑470). Single amino acid substitutions and three-amino acid deletions are associated with cardio‑skeletal myopathies. Most mutations affect filament assembly. Desmin is a highly conserved protein. Over the region used as immunogen, human Desmin is 99% identical to the corresponding mouse, canine and porcine protein sequences.

Alternate Names

CMD1I, DES

Entrez Gene IDs

1674 (Human); 13346 (Mouse); 64362 (Rat)

Gene Symbol

DES

UniProt

P17661

Additional Desmin Products

Product Documents for Human Desmin Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Desmin Antibody

For research use only

Citations
Reviews

Human Desmin Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB11654

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Desmin Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Desmin Antibody

Detection of Desmin in Human Skeletal Muscle via seqIF™ staining on COMET™