Human DcR3/TNFRSF6B Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAM2135
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA Detection (Matched Antibody Pair)
Label
Biotin
Antibody Source
Monoclonal Mouse IgG1 Clone # 229812
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human DcR3/TNFRSF6B
Val24-His300
Accession # O95407
Val24-His300
Accession # O95407
Specificity
Detects human DcR3/TNFRSF6B in ELISAs. In sandwich immunoassays, no cross-reactivity with recombinant human (rh) Fas, rhOPG, rhTNF RII, rhTRAIL RI, rhTRAIL R2, rhTRAIL R3, or rhTRAIL R4 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human DcR3/TNFRSF6B Biotinylated Antibody
Human DcR3 / TNFRSF6B ELISA Standard Curve
Recombinant Human DcR3/TNFRSF6B Fc Chimera (Catalog # 142-DC) was serially diluted and captured by Mouse Anti-Human DcR3/TNFRSF6B Monoclonal Antibody (Catalog # MAB21351) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human DcR3/TNFRSF6B Monoclonal Antibody (Catalog # MAB2135) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)Applications for Human DcR3/TNFRSF6B Biotinylated Antibody
Human DcR3/TNFRSF6B Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DcR3/TNFRSF6B
Human decoy receptor 3 (DcR3), also called TNFRSF6B, TR6 and M68, is a member of the TNF receptor superfamily. The cDNA of DcR3 encodes a 300 amino acid (aa) polypeptide with a putative 23 aa signal peptide. Like osteoprotegerin (OPG), DcR3 lacks a transmembrane sequence and is a secreted protein. DcR3 shares sequence identity with OPG (31%), TNFRII (29%) and Fas (17%). It was found to be expressed in a variety of different tissues and at high levels in many malignant tumors. Among TNF family members, DcR3 was shown to bind with Fas ligand (FasL) and LIGHT and inhibit FasL- and LIGHT-induced apoptosis. Thus, in addition to DcR1, DcR2 and OPG, DcR3 is another TNFR family molecule which modulates ligands that induce apoptosis. Overexpression of DcR3 might be a mechanism by which certain tumors escape immune-cytotoxic attack.
References
- Pitti, R.M. et al. (1998) Nature 396:699.
- Yu, K-Y. et al. (1999) J. Biochem. Chem. 274:13733.
- Bai, C. et al. (2000) Proc. Natl. Acad. Sci. USA. 97:1230.
Long Name
Decoy Receptor 3
Alternate Names
M68, TNFRSF6B, TR6
Gene Symbol
TNFRSF6B
UniProt
Additional DcR3/TNFRSF6B Products
Product Documents for Human DcR3/TNFRSF6B Biotinylated Antibody
Product Specific Notices for Human DcR3/TNFRSF6B Biotinylated Antibody
For research use only
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