Cyclin D1 was detected in immersion fixed COLO 205 human colorectal adenocarcinoma cell line using Mouse Anti-Human Cyclin D1 Monoclonal Antibody (Catalog # MAB4314) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Cyclin D1 by Western Blot

LPS suppressed EPCs cell viability via inducing cell pyroptosis. (a, b) NLRP3 was silenced in EPCs cells. (c) MTT assay was used to examine cell viability. (d) The expression status of Cyclin D1 and CDK2 were determined by Western blot analysis. Individual experiment repeated 3 times, and *P < 0.05 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34738867), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Cyclin D1 Antibody

Application

Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed COLO 205 human colorectal adenocarcinoma cell line

Reviewed Applications

Read 2 reviews rated 4 using MAB4314 in the following applications:

Immunocytochemistry (1 Review)
Immunohistochemistry (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cyclin D1

Cyclin D1 is a 44 kDa cell cycle regulatory protein whose expression level and nuclear/cytoplasmic distribution are tightly regulated in synchrony with the cell cycle. Cyclin D1 complexes containing Cdk4 or Cdk6 induce phosphorylation of Rb, a requirement for progression through the G1/S cell cycle transition. Human Cyclin D1 shares 93% amino acid sequence identity with mouse and rat Cyclin D1.

Alternate Names

Bcl-1, CCND1, Cyl-1, PRAD1

Entrez Gene IDs

595 (Human); 12443 (Mouse); 58919 (Rat)

Gene Symbol

CCND1

UniProt

P24385

Additional Cyclin D1 Products

Product Documents for Human Cyclin D1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Cyclin D1 Antibody

For research use only

Citations
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