Human CXCL13/BLC/BCA-1 Antibody Best Seller

Detection of Recombinant Human CXCL13/BLC/BCA‑1 by Western Blot.

Western blot shows 100 ng of Recombinant Human CXCL13/BLC/BCA-1 (Catalog # 801-CX) and Recombinant Mouse CXCL13/BLC/BCA-1 (Catalog # 470-BC). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human CXCL13/BLC/BCA-1 Monoclonal Antibody (Catalog # MAB801) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CXCL13/BLC/BCA-1 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Chemotaxis Induced by CXCL13/BLC/BCA-1 and Neutralization by Human CXCL13/BLC/BCA-1 Antibody.

Recombinant Human CXCL13/BLC/BCA-1 (Catalog # 801-CX) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse Anti-Human CXCL13/BLC/BCA-1 (0.05 µg/mL) is neutralized (green line) by increasing concentrations of Human CXCL13/BLC/BCA-1 Monoclonal Antibody (Catalog # MAB801). The ND₅₀ is typically 0.3-0.9 µg/mL.

Detection of Human CXCL13/BLC/BCA-1 by Flow Cytometry

Relationship of different CD8+ TRM subset with B cells and TLS in CRC tumor tissues. (A) Volcano plot showing differentially expressed genes between DP CD8+ TRM subsets and SP CD8+ TRM subsets of the GSE108989 dataset. (B) The FFPE CRC tumor tissue slides from patients with CRC were stained with DAPI (blue), CD20 (green), CD8 (yellow), CD103 (orange), and CD69 (red). (C) Relationship between the proportion of different CD8+ TRM cells and the proportion of B cells in CRC tumor tissues detected by flow cytometry (n = 40) and relationship between the proportion of different CD8+ TRM cells and the count of B cells and the count and area of TLS in CRC tumor tissues detected by mIHC (n = 64). Statistical analysis was performed by Pearson correlation analysis. (D) Schematic overview of the transwell migration assay design. (E) B cell migration rate when cultured either alone or with different CD8+ TRM subsets (n = 10) and B cell migration rate when cultured with DP CD8+ TRM cells with or without anti-CXCL13 neutralizing antibody (n = 7). Statistical analysis was performed by non-parametric Wilcoxon’s matched-pairs tests and Friedman test with Dunn’s multiple comparisons test. (*p < 0.05; **p < 0.01) Image collected and cropped by CiteAb from the following open publication (https://biosignaling.biomedcentral.com/articles/10.1186/s12964-024-0199…), licensed under a CC-BY license. Not internally tested by R&D Systems.