Human CXCL12/SDF-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF-310-NA
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse, Zebrafish
Applications
Validated:
Western Blot, Neutralization
Cited:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Neutralization, Immunocytochemistry, Bioassay, Blocking/Neutralizing, ELISA Development (Capture), Functional Assay
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human CXCL12/SDF‑1 alpha
Lys22-Lys89
Accession # Q6ICW0
Lys22-Lys89
Accession # Q6ICW0
Specificity
Detects human CXCL12/SDF-1 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human CXCL12/SDF-1 Antibody
Chemotaxis Induced by CXCL12/SDF-1 alpha and Neutralization by Human CXCL12/SDF-1 Antibody.
Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF-1a (Catalog # 350-NS) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF-1a (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CXCL12/SDF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-310-NA). The ND50 is typically 1.5-6.0 µg/mL.
Human CXCL12 / SDF-1 ELISA Standard Curve
Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF-1 alpha (Catalog # 350-NS) was serially diluted and captured by Mouse Anti-Human/Mouse CXCL12/SDF-1 Monoclonal Antibody (Catalog # MAB350) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CXCL12/SDF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-310-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)
Mouse CXCL12 / SDF-1 ELISA Standard Curve
Recombinant Mouse CXCL12/SDF-1 alpha (Catalog # 460-SD) was serially diluted and captured by Mouse Anti-Human/Mouse CXCL12/SDF-1 Monoclonal Antibody (Catalog # MAB350) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CXCL12/SDF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-310-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)Applications for Human CXCL12/SDF-1 Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human/Feline CXCL12/SDF-1 beta aa 19-93 (Catalog # 2716-SD)
Recombinant Human/Feline CXCL12/SDF-1 beta aa 22-93 (Catalog # 351-FS)
Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF-1 alpha (Catalog # 350-NS)
Sample: Recombinant Human/Feline CXCL12/SDF-1 beta aa 19-93 (Catalog # 2716-SD)
Recombinant Human/Feline CXCL12/SDF-1 beta aa 22-93 (Catalog # 351-FS)
Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF-1 alpha (Catalog # 350-NS)
Neutralization
Measured by its ability to neutralize CXCL12/SDF-1 alpha-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CXCR4. The Neutralization Dose (ND50) is typically 1.5-6.0 µg/mL in the presence of 2 ng/mL Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF-1 alpha.
Reviewed Applications
Read 2 reviews rated 4 using AF-310-NA in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCL12/SDF-1
CXCL12, also known as SDF-1 (stromal cell-derived factor 1) and PBSF (Pre-B cell growth stimulatory factor), is a member of the CXC chemokine superfamily. Two alternatively spliced mRNAs encode CXCL12 alpha (89 aa residues) and CXCL12 beta (93 aa residues) that differ by the addition of 4 amino acids at the carboxy-terminus. Human and mouse CXCL12 are highly conserved. Mature human and mouse CXCL12 differ only by 1 amino acid.
Alternate Names
PBSF, SDF-1, SDF1
Gene Symbol
CXCL12
UniProt
Additional CXCL12/SDF-1 Products
Product Documents for Human CXCL12/SDF-1 Antibody
Product Specific Notices for Human CXCL12/SDF-1 Antibody
For research use only
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