Detects human CX3CL1 in ELISAs and Western blots. In ELISAs, this antibody shows approximately 8% cross-reactivity with recombinant mouse (rm) CXCL6 and less than 0.2% cross-reactivity with rhCXCL6, rhCXCL9, and rrCX3CL1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human CX3CL1/Fractalkine Chemokine Domain Biotinylated Antibody

Human CX3CL1 / Fractalkine ELISA Standard Curve

Recombinant Human CX3CL1/Fractalkine Full Length (Catalog # 365-FR) was serially diluted and captured by Mouse Anti-Human CX3CL1/Fractalkine Chemokine Domain Monoclonal Antibody (Catalog # MAB3652) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human CX3CL1/Fractalkine Chemokine Domain Monoclonal Antibody (Catalog # MAB365) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human CX3CL1/Fractalkine Chemokine Domain Biotinylated Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human CX3CL1/Fractalkine Full Length (Catalog # 365-FR)

Human CX3CL1/Fractalkine Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)

Recommended Concentration: 0.5-2.0 µg/mL
Use in combination with these reagents:

Capture Reagent: Human CX3CL1/Fractalkine Chemokine Domain Antibody (Catalog # MAB3652)
Standard: Recombinant Human CX3CL1/Fractalkine (Full Length) Protein (Catalog # 365-FR)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CX3CL1/Fractalkine

Fractalkine is a type I membrane protein in which a chemokine domain possessing a unique C-X3-C cysteine motif is tethered on a long mucin-like stalk. It can also be released as a soluble molecule upon proteolysis at a membrane proximal site.

Alternate Names

FKN, Fractalkine, Neurotactin

Entrez Gene IDs

6376 (Human); 20312 (Mouse); 89808 (Rat); 102117496 (Cynomolgus Monkey)

Gene Symbol

CX3CL1

UniProt

P78423

Additional CX3CL1/Fractalkine Products

Product Documents for Human CX3CL1/Fractalkine Chemokine Domain Biotinylated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human CX3CL1/Fractalkine Chemokine Domain Biotinylated Antibody

For research use only

Citations
Reviews

Human CX3CL1/Fractalkine Chemokine Domain Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAM365

Key Product Details

Species Reactivity

Validated:

Cited:

Applications