Human Coronavirus Spike Protein Antibody (07) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06647
![Western Blot: Human Coronavirus Spike Protein Antibody (07) [NBP3-06647]](https://resources.bio-techne.com/images/products/Human-Coronavirus-Spike-Protein-Antibody-07-Western-Blot-NBP3-06647-img0001.jpg "Western Blot: Human Coronavirus Spike Protein Antibody (07) [NBP3-06647]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
HCoV-HKU1
Applications
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 07
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant HCoV-HKU1 (Accession#: YP_173238.1; Met 1-Arg 760).
Reactivity Notes
No cross-reactivity in ELISA with:
Human cell lysate (293 cell line)
Human cell lysate (293 cell line)
Specificity
HCoV-HKU1 (isolate N1) Spike/S1No cross-reactivity in ELISA with:HCoV-HKU1 (isolate N5) Spike S1+S2 ECD ProteinSARS-CoV-2 Spike S1 ProteinSARS-CoV-2 Spike S1 NTD ProteinSARS-CoV-2 Spike S1 NTD ProteinSARS-CoV-2 Spike RBD ProteinSARS-CoV-2 Spike S2 ProteinSARS-CoV-2 Spike S1+S2 ECD ProteinSARS-CoV Spike S1+S2 ECD (S577A, Isolate Tor2) ProteinMERS-CoV Spike S1+S2 ECD ProteinHCoV-NL63 Spike S1+S2 ECD ProteinHCoV-229E Spike S1+S2 ECD ProteinHCoV-OC43 Spike S1+S2 ECD Protein
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for Human Coronavirus Spike Protein Antibody (07) - Azide and BSA Free
Western Blot: Human Coronavirus Spike Protein Antibody (07) [NBP3-06647]
Western Blot: Human Coronavirus Spike Protein Antibody (07) [NBP3-06647] - Human coronavirus HKU1 (isolate N1) (HCoV-HKU1) Spike/S1 Protein. Lane A: 100ng, Lane B: 20ng. SecondaryGoat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Applications for Human Coronavirus Spike Protein Antibody (07) - Azide and BSA Free
Application
Recommended Usage
ELISA
1:1000-1:2000
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.0)
Format
Azide and BSA Free
Preservative
10% Trehalose
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Human Coronavirus Spike Protein
Alternate Names
HKU1, Spike glycoprotein
Gene Symbol
S
Additional Human Coronavirus Spike Protein Products
Product Documents for Human Coronavirus Spike Protein Antibody (07) - Azide and BSA Free
Product Specific Notices for Human Coronavirus Spike Protein Antibody (07) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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