Human Common gamma Chain/IL-2 R gamma APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB2842A
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Flow Cytometry
Cited:
Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Mouse IgG2B Clone # 633162
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf21-derived recombinant human Common gamma Chain/IL-2 gamma
Leu23-Asn254
Accession # P31785
Leu23-Asn254
Accession # P31785
Specificity
Detects human Common gamma Chain/IL-2 R gamma in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-2 R alpha, rhIL-2 R beta, rhIL-15 R alpha, recombinant mouse IL-2 R gamma, or recombinant canine IL-2 R alpha is observed. In Western blots, approximately 80% cross-reactivity with rhIL-2 R beta, 50% cross-reactivity with rhIL-15 R alpha and recombinant mouse IL-2 R alpha, and no cross-reactivity withrhIL-2 R alpha is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images
Detection of Common gamma Chain/IL‑2 R gamma in Human Blood Lymphocytes by Flow Cytometry.
Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3e PE-conjugated Monoclonal Antibody (Catalog # FAB100P) and either (A) Mouse Anti-Human Common gamma Chain/IL-2 R gamma APC-conjugated Monoclonal Antibody (Catalog # FAB2842A) or (B) Mouse IgG2BAllophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.Applications
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: Human peripheral blood lymphocytes
Sample: Human peripheral blood lymphocytes
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
Background: Common gamma Chain/IL-2 R gamma
The gamma chain of the high affinity functional human IL-2 receptor complex belongs to the hematopoietin receptor family. IL-2 R gamma is a 369 amino acid protein consisting of a 22 aa signal sequence, a 232 aa extracellular domain, a 29 aa transmembrane domain and an 86 aa cytoplasmic domain. Although IL-2 R gamma by itself does not bind IL‑2 with any appreciable affinity, it is required for IL-2 receptor signaling. Besides IL-2, the gamma chain has been shown to be a component of the functional receptor complexes for IL-4, IL-7, IL-9 and IL-15. IL-2 R gamma has been designated the common gamma chain ( gammac). The site of molecular defects in X-linked SCID (severe combined immunodeficiency) has been mapped to the IL-2 R gamma gene.
References
- Minami, Y. et al. (1993) Annu. Rev. Immunol. 11:245.
- Noguchi, M. et al. (1993) Science 262:1877.
Long Name
IL-2 Receptor gamma
Alternate Names
CD132, IL-2 R gamma, IL2R gamma, IL2RG
Gene Symbol
IL2RG
UniProt
Additional Common gamma Chain/IL-2 R gamma Products
Product Specific Notices
For research use only
Loading...
Loading...
Loading...
Loading...
Loading...