Human CEBP  alpha Alexa Fluor™ Plus 647-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB7094AFP647

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FAB7094AFP647-100UG
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry

Label

Alexa Fluor Plus 647 (Excitation = 658 nm, Emission = 675 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 692716

View all CEBP alpha Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CEBP alpha

Specificity

Detects human CEBP alpha in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human CEBP beta, gamma, delta, epsilon, or zeta is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications

Application
Recommended Usage

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: CEBP alpha

CCAAT-enhancer binding protein alpha (CEBP alpha) is a widely expressed 42 kDa basic-leucine zipper transcription factor that forms functional homodimers and heterodimers with CEBP beta and CEBP gamma. CEBP alpha inhibits mitotic progression through multiple mechanisms and also promotes terminal differentiation of granulocytes and adipocytes. Its activity is regulated by SUMOylation and by phosphorylation at multiple serine residues. CEBP alpha contains three TE transactivation domains (aa 1‑69, aa 70-96, and aa 126-200) and one basic DNA binding motif and leucine zipper (aa 286 - 345). A 30 kDa isoform is generated by the use of an internal translation initiation site. The p30 isoform functions as a dominant negative factor by heterodimerizing with and inhibiting full length CEBP alpha activity. Within aa 1‑124, human CEBP alpha shares 93% amino acid sequence identity with mouse and rat CEBP alpha, respectively.

Long Name

CCAAT/enhancer-Binding Protein alpha

Alternate Names

C/EBP-alpha, CEBPA

Entrez Gene IDs

1050 (Human); 12606 (Mouse); 24252 (Rat)

Gene Symbol

CEBPA

UniProt

P49715 (Human)

Additional CEBP alpha Products

Product Documents

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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