Human peripheral blood cells were stained with Human CD96 Monoclonal Antibody (Catalog # MAB6199) followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B) and Human CD3e Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB100F). Quadrant markers were set based on isotype control antibody staining (Catalog # MAB002).

Applications for Human CD96v2 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: Human peripheral blood cells

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD96

CD96 (also known as Tactile) is a 160 kDa type I transmembrane glycoprotein that contains three Ig-like domains in its extracellular region. It is expressed on CD4⁺ and CD8⁺ T cells, NK cells, and select B cells. CD96 binds to CD155/PVR and participates in NK cell-mediated lysis of CD155⁺ target cells. Alternate splicing generates a short variant (CD96v2) which lacks 16 amino acids (aa) within the second Ig-like domain. CD96v2 is the predominant isoform in many cell types and exhibits even greater binding affinity with CD155 than does full length CD96. A soluble form of CD96 circulates in the serum. Over aa 22-501, human CD96v2 shares 55% aa sequence identity with mouse and rat CD96.

Alternate Names

CD96, TACTILE

Entrez Gene IDs

10225 (Human); 84544 (Mouse); 498079 (Rat); 102121080 (Cynomolgus Monkey)

Gene Symbol

CD96

UniProt

NP_005807

Additional CD96 Products

Product Documents for Human CD96v2 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human CD96v2 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human CD96v2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6199

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all CD96 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human CD96v2 Antibody

Detection of CD96 in Human PBMCs by Flow Cytometry.