Human CD31/PECAM-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF806
Conjugate
Catalog #
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human
Cited:
Human, Mouse, Bovine, Primate, Primate - Macaca fuscata (Japanese Macaque), Primate - Macaca mulatta (Rhesus Macaque), Rabbit, Transgenic Mouse, Xenograft
Applications
Validated:
Knockout Validated, Immunohistochemistry, Western Blot, Immunocytochemistry, Simple Western
Cited:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry, ELISA Capture, FACS, Flourescense Microscopy
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human CD31
Extracellular domain
Extracellular domain
Specificity
Detects human CD31 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse CD31 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human CD31/PECAM-1 Antibody
Detection of Human CD31/PECAM‑1 by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF806) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for CD31/PECAM-1 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
CD31/PECAM‑1 in HUVECs.
CD31/PECAM-1 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using 10 µg/mL Sheep Anti-Human CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF806) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human CD31/PECAM-1 by Simple WesternTM.
Left: Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells, loaded at 0.1 mg/ml. A specific band was detected for CD31/PECAM-1 at approximately 155 kDa (as indicated) using both 10 µg/ml and 50 µg/ml of Sheep Anti-Human CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF806) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016) in Milk-free Antibody Diluent (Catalog # 043-524). This experiment was conducted under reducing conditions and using the 12-230kDa separation system. Right: Simple Western electropherogram showing the same Sheep Anti-Human CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF806) tested at 10 µg/ml (blue line) and 50 µg/ml (green line) in the HUVEC human umbilical vein endothelial cells.
Applications for Human CD31/PECAM-1 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed HUVEC human umbilical vein endothelial cells
Sample: Immersion fixed HUVEC human umbilical vein endothelial cells
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver
Sample: Immersion fixed paraffin-embedded sections of human liver
Knockout Validated
CD31/PECAM‑1 was detected in immersion fixed THP‑1 human acute monocytic leukemia cell line but is not detected in CD31/PECAM‑1 knockout (KO) THP‑1 human cell line.
Simple Western
10-50 µg/mL
Sample: HUVEC human umbilical vein endothelial cells
Sample: HUVEC human umbilical vein endothelial cells
Western Blot
1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line
Sample: HepG2 human hepatocellular carcinoma cell line
Reviewed Applications
Read 3 reviews rated 4.7 using AF806 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD31/PECAM-1
The CD31 adhesion molecule, also known as PECAM-1, is expressed in large amounts on endothelial cells at intercellular junctions and on T cell subsets, and to a lesser extent on platelets and most other leukocytes such as monocytes and neutrophils. CD31 binds to itself homotypically, and also to the leukocyte integrin alphav beta3 heterotypically. CD31 is required for the transendothelial migration of leukocytes through intercellular junctions of vascular endothelial cells. CD31 has been found in human plasma, and the presence of this circulating isoform is suggested to modulate the transendothelial migration of leukocytes.
Long Name
Platelet Endothelial Cell Adhesion Molecule 1
Alternate Names
CD31, EndoCAM, PECA1, PECAM-1, PECAM1
Gene Symbol
PECAM1
Additional CD31/PECAM-1 Products
Product Documents for Human CD31/PECAM-1 Antibody
Product Specific Notices for Human CD31/PECAM-1 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...