Human CD31/PECAM-1 Antibody

Detection of CD31 in Human Prostate Cancer via seqIF™ staining on COMET™

CD31 Antibody was detected in immersion fixed paraffin-embedded sections of human Prostate Cancer using Rabbit Anti-Human CD31, Monoclonal Antibody (Catalog #MAB11678) at 8ug/mL at 37° Celsius for 2 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 555 Goat anti-Rabbit IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane of endothelial cells. Protocol available in COMET™ Panel Builder.​​

Detection of Human CD31/PECAM-1 by Western Blot.

Western Blot shows lysates of THP-1 human acute monocytic leukemia cell line. PVDF membrane was probed with 1 µg/ml of Rabbit Anti-Human CD31/PECAM-1 Monoclonal Antibody (Catalog # MAB11678) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for CD31/PECAM-1 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of CD31/PECAM-1 in Human Colon.

CD31/PECAM-1 was detected in immersion fixed paraffin-embedded sections of human colon using Rabbit Anti-Human CD31/PECAM-1 Monoclonal Antibody (Catalog # MAB11678) at 3 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003) or the HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.