Human CD20 mFluor™ Violet 450-Conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB4225MFV450

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FAB4225MFV450-100
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

mFluor Violet 450 SE (Excitation = 406 nm, Emission = 445 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 396444

View all CD20 Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human CD20
Met1-Pro297
Accession # P11836

Specificity

Detects human CD20. Stains human CD20 transfectants but not irrelevant transfectants.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human CD20 mFluor™ Violet 450-Conjugated Antibody

CD20 Antibody (396444) [mFluor Violet 450 SE]

Detection of CD20 in PBMC lymphocytes by Flow Cytometry

PBMC lymphocytes were stained with Mouse Anti-Human CD19 PE-conjugated Monoclonal Antibody (Catalog # FAB4867P) and either (A) Mouse Anti-Human CD20 mFluor™ Violet 450-Conjugated Monoclonal Antibody (Catalog # FAB4225MFV450) or (B) unstained . View our protocol for Staining Membrane-associated Proteins.

Applications for Human CD20 mFluor™ Violet 450-Conjugated Antibody

Application
Recommended Usage

Flow Cytometry

0.1-1 µg/106 cells
Sample: PBMC lymphocytes

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD20

CD20 is a 297 amino acid (aa) non-glycosylated type III membrane protein that is the founding member of the MS4A tetraspanin protein family. It is expressed on pre-B, naïve and mature B lymphocytes and B-cell lymphomas. CD20 is associated with lipid rafts upon crosslinking, and appears to play a role in regulation of ion influx. The one extracellular segment of 48 aa shows 65% aa sequence identity between human and mouse CD20.

Long Name

Cluster of Differentiation 20

Alternate Names

B1, Bp35, CD20, LEU-16, Ly-44, MS4A1, S7

Entrez Gene IDs

931 (Human); 12482 (Mouse); 25316 (Rat); 485430 (Canine); 102118491 (Cynomolgus Monkey)

Gene Symbol

MS4A1

UniProt

P11836

Additional CD20 Products

Product Documents for Human CD20 mFluor™ Violet 450-Conjugated Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human CD20 mFluor™ Violet 450-Conjugated Antibody

mFluorTM is a trademark of AAT Bioquest, Inc.

For research use only

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