Human CD20 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB42251
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 1027943
Product Specifications
Immunogen
Human CD20 Peptide. The epitope recognized by rituximab
Binder M, et al. (2006) 108:1975-8. doi: 10.1182/blood-2006-04-014639. Epub 2006 May 16. PMID: 16705086.
Specificity
Detects human CD20 in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human CD20 Antibody
Detection of Human CD20 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HEK293T human embryonic kidney cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CD20 Monoclonal Antibody (Catalog # MAB42251) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for CD20 at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.Applications for Human CD20 Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and HEK293T human embryonic kidney cell line
Sample: HeLa human cervical epithelial carcinoma cell line and HEK293T human embryonic kidney cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD20
CD20 is a 297 amino acid (aa) non-glycosylated type III membrane protein that is the founding member of the MS4A tetraspanin protein family. It is expressed on pre-B, naïve and mature B lymphocytes and B-cell lymphomas. CD20 is associated with lipid rafts upon crosslinking, and appears to play a role in regulation of ion influx. The one extracellular segment of 48 aa shows 65% aa sequence identity between human and mouse CD20.
Long Name
Cluster of Differentiation 20
Alternate Names
B1, Bp35, CD20, LEU-16, Ly-44, MS4A1, S7
Entrez Gene IDs
Gene Symbol
MS4A1
Additional CD20 Products
Product Documents for Human CD20 Antibody
Product Specific Notices for Human CD20 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...