Human peripheral blood mononuclear cells (PBMCs) were stained with Recombinant Mouse Anti-Human CD3e Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB100N) and either (A) Mouse Anti-Human CD19 Monoclonal Antibody (Catalog # MAB4867R) or (B) Mouse IgG₁Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Applications for Human CD19 Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Human peripheral blood mononuclear cells (PBMCs)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD19

CD19 is a 95 kDa transmembrane glycoprotein with two Ig-like C2-set domains. CD19 regulates B cell development and activation through interactions with CD21, CD22, and the B cell receptor. CD19 polymorphisms and up‑regulation lead to the development of autoimmunity by promoting autoantibody production. Within the extracellular domain, human CD19 (Accession # P15391) shares 57% amino acid sequence identity with mouse and rat CD19.

Alternate Names

CD19, CVID3, Leu-12

Entrez Gene IDs

930 (Human); 12478 (Mouse); 365367 (Rat); 397669 (Porcine); 607898 (Canine); 102145514 (Cynomolgus Monkey); 100147468 (Equine)

Gene Symbol

CD19

Additional CD19 Products

Product Documents for Human CD19 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human CD19 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human CD19 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4867R

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all CD19 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human CD19 Antibody

Detection of CD19 in Human PBMCs by Flow Cytometry.